The overall aim of this study was to investigate the effect of, and the interaction between, the diurnal and circamensual variation on cardiorespiratory system. Twenty eumenorrheic females (age 21.5±2.5 years, height 1.64±1.03 m and weight 57.2±4.8 kg: mean±SD) took part in this study. All subjects reported a history of normal menstrual cycle lasting 26-32 days and none had used any form of oral contraceptives and Hormonal drugs for at least 4 month before entering the study. To deal with a possible “ first experiment effect”, all subject underwent two familiarization session in order to minimize learning effects and increase reliability of measurements as has been done in some proceeding work to the experimental work. For main testing, subjects attended the laboratory at 06:00 h and 18:00 h during the two phases (follicular and luteal) of the menstrual cycle. Body temperature was measured during 30 min before the tests in the standard position. Cardiorespiretory indicators (heart rate and blood pressure) was measured before and after activity. In addition, maximal oxygen consumption (vo2max) was assessed by Bruce incremental treadmill. Two way repeated measures ANOVA (time of day×cycle phase) was used to examine the effect of time of day and phases of the menstrual cycle and interaction effects of circadian rhythm and menstrual cycle on Cardiorespiretory Functions . Also 1- Sample KS test was used to normality of distribution. Body temperature increased significantly from 06:00 to 18:00 by 0.8oC (F=183.43, P=0.000). There was found a significant difference in time of day on Vo2max (F=38.71, P=0.000), at 18:00 was 2.6 ml/min greater than 06:00 h. A time of day effect also was significant for heart rate before (F=42.01, P=0.000) and after (F=10.32, P=0.005) activity which at 18:00 h was respectively 8, 4 rate per min greater than 06:00 h. Diurnal variation also was observed for systolic blood pressure before (F=16.04, P=0.001) and after (F=6.09, P=0.023) activity which at 18:00 h was respectively 6, 4.8 ml. hg lesser than at 06:00h. A significant circamensual variation was observed for body temperature (F=38.97, P=0.000), heart rate before (F=12.59, P=0.002) and after (F=5.51, P=0.030) activity with values greater in luteal phase. In addition, vo2max (F=59.62, P=0.000), and systolic blood pressure before (F=9, P=0.007) and after (F=5.13, P=0.035) activity showed circamensual variation, but values were greater at follicular compared to luteal phase. Interaction effects between time of day and menstrual phase was observed just on body temperature (F=37.13, P=0.000). The present results suggest that female’s cardiorespiratory indicators has been affected by time of day and menstrual cycle phases. Therefore diurnal and circamensual variation should be taken into account when measuring body temperature and cardiorespiratory indicators. Therefore, in studies of females should be carefully controled circadian rhythm and circamensual variation.