Background: Self-medication with diet supplements has been increased. Media advertisements have an important role in this regard.Objectives: The aim of this study was to determine the status of media literacy and attitudes about self-medication with diet supplements for weight loss in dormitory students of Iran University of Medical Sciences, Iran.Materials and Methods: In this cross-sectional study, 98 dormitory students of Iran University of Medical Sciences were selected using random method. Data collection tools were two researcher-made questionnaires about attitudes towards self-medication with diet supplements and media literacy, and two standard questionnaires including Body Image Concern Inventory (BICI) and Coppersmith Self-Esteem Inventory (SEI). Data were collected through questionnaires and interviews. Data were analyzed using SPSS software version 16, descriptive statistics (frequency, percentage, mean and standard deviation), and analytical tests (Pearson’s correlation coefficient, Spearman’s correlation coefficient, chi-squared and Fisher’s exact tests).Results: The status of attitudes and dimensions of media literacy (knowledge, critical thinking and analysis of media) was moderate. There were correlations in the mean score of attitude with media management awareness, critical thinking, and analysis (r = 0.99, r = 0.71 and r = 0.57, respectively; in each case, P=0.0001). Postgraduate students had higher scores of attitude, self-esteem, awareness, media management, critical thinking and analysis and lower scores of body image concern.Discussion and Conclusion: The states of attitudes, media literacy, body image and self-esteem in studied students were moderate and promotional interventions in this area are recommended.