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مرکز اطلاعات علمی SID1
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Issue Info: 
  • Year: 

    2020
  • Volume: 

    8
  • Issue: 

    2 (30)
  • Pages: 

    1-12
Measures: 
  • Citations: 

    0
  • Views: 

    708
  • Downloads: 

    674
Abstract: 

Nepeta crispa Willd. Locally known as Mofarrah belongs to Lamiaceae family. It is an Iranian endemic self-growing medicinal plant to Alvand Mountains in Hamadan province. N. crispa has carminative, anti-inflammatory, antinociceptive, antibacterial and antifungal properties. The aim of this study was to investigate the phytochemical composition of essential oil compounds of this plant in the vegetative stage, which cultivated in Tarbiat Modares University of Tehran at an altitude of 1283 m above sea level, and compare with samples taken from natural habitats in Arzanfood and Gashani with the altitude of 3015 and 2489 m above sea level, respectively. The plants were harvested during the late July 2018. The essential oils of all samples were obtained by hydrodistillation and were analyzed by GC-MS and GC-FID. The yield of essential oil in the sample collected from Arzanfood, Gashani and field samples were 2. 13, 1. 97 and 0. 55% (w/w), respectively. Twenty components were identified among the samples, representing 93-96% of the oils. The main components were 1, 8-cineole (40. 25%, 48. 5, 49. 9%), 4aα , 7β , 7aα-nepetalactone) 34. 8%, 3. 7%, 24. 7%) and β-pinene) 6. 2%, 5. 4%, 5. 4%) for cultivated field, Gashani and Arzanfood samples, respectively. Although the percentage of oil and 1, 8-cineole in the cultivated sample were low, but the amount of nepetalactome compounds was high. According to the results, the environmental conditions had an important role in the qualitative and quantitative changes in the essential oil of this plant but cultivation of N. crispa in a place with more than 1200 m difference in altitude, lower than the main habitat, was a promising sign for domestication of N. crispa. Optimization of other factors including, cultivation in an area close to the natural habitat, nutrition, biotic and abiotic stresses treatment could even improve the quality of this plant compared to the self-growing ones.

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Issue Info: 
  • Year: 

    2020
  • Volume: 

    8
  • Issue: 

    2 (30)
  • Pages: 

    13-29
Measures: 
  • Citations: 

    0
  • Views: 

    292
  • Downloads: 

    439
Abstract: 

Satureja hortensis L. is one of the valuable plants belonging to the Lamiaceae family that its leaves and flowering branches contain essential oil. The aim of this study was to evaluate the effects of Thiobacillus biological and chemical fertilizers on morphological and phytochemical characteristics of this plant. The present study was carried out as a factorial experiment based on randomized complete block design with 3 replications in the research farm of Golmakan town in 2019. Experimental treatments included Thiobacillus biological fertilizer (0, 5 and 12 kg/ha) and chemical fertilizer (0, 35 and 70 kg/ha). Plant essential oil was extracted from savory leaves at flowering stage by Clevenger apparatus, then the constituents of the essential oil were analyzed by GC-MS. The results showed that all morphological traits of the plant as well as the quality and quantity of the active ingredients of the essential oil were affected by fertilizer treatments. The combined application of biological and chemical fertilizers led to an increase in the studied traits compared to the non-application of fertilizer. The highest plant height (48. 8 cm), leaf dry weight (9. 7 g) and essential oil density (3. 67%) were obtained in the combined use of fertilizers. The two main constituents of savory essential oil including carvacrol (62. 10%) and gamaterpinen (19. 04%) showed the highest increase in the treatment composition of 12 kg/ha of Thiobacillus fertilizer and 70 kg/ha of chemical fertilizer (NPK). Based on the results, we found that combinations of biofertilizer and chemical fertilizer could increase the morphological characteristics, quantity and quality of savory essential oil.

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Author(s): 

Moradkhani Sakineh

Issue Info: 
  • Year: 

    2020
  • Volume: 

    8
  • Issue: 

    2 (30)
  • Pages: 

    30-44
Measures: 
  • Citations: 

    0
  • Views: 

    393
  • Downloads: 

    498
Abstract: 

Fig fruit (Ficus carica L. ) contains large amounts of phenolic-compounds, flavonoids and anthocyanins, which are the most important natural antioxidants. In this study, the fruit of three fig genotypes from three villages of Qerkh-Yashar, Badal-Abad and Pirmousi located in Khoy city, West-Azarbaijan province was harvested in September of 2019 and their biochemical characteristics were investigated. Some important chemical properties of the fruit were measured, such as antioxidant capacity with DPPH method, total phenol-content, total anthocyanin with Spectrophotometer, soluble sugars and polyphenols with HPLC. The measured antioxidant capacity by DPPH method in fruits varied from 36. 93 to 45. 67%. The highest total phenol was 418. 59 mg in 100g of fresh gallic-acid, which was observed in the second genotype. The highest anthocyanin was 1. 219 mg in 100g of fresh weight, which was observed in the first genotype. According to the results of the analysis of soluble sugars in all three sugars: fructose, sucrose and glucose were observed in all three genotypes. in this study, 9 types of polyphenolic-compounds were extracted from fig fruit, which included: caffeic-acid, gallic-acid, chlorogenic-acid, rutin, coumaric, rosemary-acid, quercetin, cinnamic-acid, apagenine, and the combination of chlorogenic-acid with an average of 30. 88 micrograms-pergram was identified as the dominant polyphenol. According to the results of this study, different fig genotypes contain antioxidants and natural polyphenols, among which the first genotype, which belongs to the village of Qerkh-Yashar, contains higher levels of antioxidants and Polyphenols can be suggested for future correctional programs, but can also be used in the food and pharmaceutical industries.

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Issue Info: 
  • Year: 

    2020
  • Volume: 

    8
  • Issue: 

    2 (30)
  • Pages: 

    45-59
Measures: 
  • Citations: 

    0
  • Views: 

    323
  • Downloads: 

    470
Abstract: 

The fruit of Rosa canina L. contains valuable substances such as fatty acids. In this study, in order to determine the amount of oil and fatty acid profiles of Rosa canina in different habitats of Iran and to know the diversity of these compounds, the fruits at full ripening stage were collected from 5 habitats with different climatic conditions, including Urmia, Chaldran, Bukan, Shahindeg and Zanjan with a height of 1500, 2000, 1370, 1406 and 1638 m above sea level, respectively, on 21 September in 2017. The oil was extracted from the seeds with Soxhlet apparatus and fatty acids were analyzed by gas chromatography (GC). The results were showed that different habitats had a significant impact on the oil content and fatty acids profiles. The highest and lowest percentages of oil were obtained from Shahindeg (20. 57 %) and Chaldran (12. 37 %) habitats, respectively. The predominant fatty acids in seed oil of Chaldran habitat were palmitic acid (49. 53 %), linoleic acid (46. 71 %), cis-9-oleic acid (28. 81 %) and cis-11-eicosanoic acid (17. 88 %), meanwhile in the Urmia habitat; they were linoleic acid (42. 96 %), cis-9-oleic acid (30. 33 %), cis-11-eicosanoic acid (15. 34 %) and palmitic acid (5. 92 %). In the Zanjan habitat, the major of fatty acids were including linoleic acid (46. 30 %), cis-9-oleic acid (28. 42 %), cis-11-eicosanoic acid (16. 71 %) and palmitic acid (4. 54 %). In the Bukan habitat, the predominant fatty acids were linoleic acid (45. 31 %), cis-9-oleic acid (27. 70 %), cis-11-eicosanoic acid (17. 70 %) and palmitic acid (5. 33 %), while in the Shahindeg habitat; they were linoleic acid (52. 61%), cis-9 oleic acid (28. 62 %) and cis-11-eicosanoic acid (18. 70 %). The Urmia and Bukan habitats had the highest amount of saturated fatty acids, while the seeds of Shahindeg habitat were rich in unsaturated fatty acids. In general, the results of this study proved the effect of ecological conditions on the quantity and quality of fatty acids and the percentage of oil in seed of Rosa canina L.

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Issue Info: 
  • Year: 

    2020
  • Volume: 

    8
  • Issue: 

    2 (30)
  • Pages: 

    60-75
Measures: 
  • Citations: 

    0
  • Views: 

    621
  • Downloads: 

    576
Abstract: 

M. pulegium is belongs to Lamiaceae. Pulegon as a main ingredient in mint has antimicrobial and aromatic properties which has been used to treatment of many diseases. In this study M. pulegium were collected from three natural habitats of Ziaber, Masal and Abkenard in the flowering stage in Guilan province. Also, rhizomes of habitat samples were cultivated in the research farm of Zanjan University in the same year. The rhizomes of the these populations were collected in the spring of 2017 and transferred to the research farm of Zanjan University. In the summer of the same year, flowering branches were harvested from natural habitates. The essential oil was extracted by using a Clevenger apparatus and water distillation and were and bused by GC and GC-MS. Results were showed that the polygon were highly varied in all populations, in which the highest and lowest content of pulegone was observed in cultivated population in Zanjan and Abkenar population with 54. 43% and 40. 19%, respectively. According to the results, the populations grown habitat were superior to morphological traits such as leaf length, plant height and number of internodes. A higher percentage of essential oil was observed in the cultivated population, it may be due to decrease in rainfall, an increase in altitude, as well as differences in latitude and longitude. The pulegon and camphene in Ziaber region had the highest amount with 49. 17% and 11. 84%, respectively, in Masal region, pulegon with 49. 47% and gamaterpinen with 24. 24% and in Abkenar region, pulegon (40. 19%) and gamaterpinen (11. 56%) were the most compounds. The high amount of these compounds in the studied populations indicates the high quality of essential oil in this medicinal plant. In this study, the cultivated populations had fewer compounds with a high percentage of the main compounds compared to other populations, so it seems they have more superiority and potential for breeding and domestication programs.

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Issue Info: 
  • Year: 

    2020
  • Volume: 

    8
  • Issue: 

    2 (30)
  • Pages: 

    76-88
Measures: 
  • Citations: 

    0
  • Views: 

    476
  • Downloads: 

    450
Abstract: 

The aim of this study was to evaluate the effect of elicitors on antioxidant properties and mycelial secondary metabolites of Arthrinium arundinis, Lichen symbiont, and Hyphodontia paradoxa and Stereum hirsutum hosted by Betula pendula. In the summer of 2016 preliminary sampling was performed from tree barks of Siah-Marzkouh, Golestan province, from an altitude of 2400 m above sea level. Then, fungal purification was achieved. In 2018, biennial mycelial reserves were used to investigate changes in secondary compounds. The following treatments were applied as elicitor: sodium nitrate, nanofibers cellulose and magnesium sulphate. Initial evaluation of triterpenoids was performed by HPLC using standard betulin and betulinic acid, and LC-MS was used to confirm the corresponding peak as triterpenoid. Antioxidant properties in mycelia of these fungi were studied by DPPH method. Based on the results, the highest peak of triterpenoids which are derivatives of betulin was in H. paradoxa treated by nanofibers cellulose, while in S. hirsutum it was by sodium nitrate (6. 5 and 6. 37 times in comparison to control, respectively). In A. arundinis, elicitors had no effect on triterpenoids amount and significantly reduced it. The antioxidant activity of H. paradoxa in the control treatment was 83. 1% and the induction of elicitors had the opposite effect on this activity. The antioxidant activity of A. arundinis decreased from 82. 05 to 87. 12% with magnesium sulfate elicitor, meanwhile by adding sodium nitrate to the medium it increased from 24. 62 to 86. 04%.

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Issue Info: 
  • Year: 

    2020
  • Volume: 

    8
  • Issue: 

    2 (30)
  • Pages: 

    89-111
Measures: 
  • Citations: 

    0
  • Views: 

    384
  • Downloads: 

    265
Abstract: 

The present study is the first report on the phytosynthesis of silver nanoparticles from aqueous extract of Salvia rhytidea Benth and comparison of its antimicrobial Activities with natural plant extract. Plant samples were collected at the flowering stage in June 2017 from the heights of Taftan Mountain (length 60 degrees and 44 minutes east longitude and width 28 degrees and 33 minutes north and at an altitude of 1394 m above sea level). After preparation of extract by maceration method, the silver nanoparticles were biosynthesized. Then, parameters such as reaction pH, concentration of AgNO3 and reaction time were studied and optimized. After characterization of nanoparticles by transmission electron microscopy (TEM) and X-ray diffraction (XRD) techniques, their antimicrobial activity against Salmonella typhimurium, Escherichia coli, Bacillus cereus and Staphylococcus aureus, Aspergilus Flavus and Penicillium expansum were investigated and compared with aquatic extract by Disk diffusion method and MIC test. The results showed that silver nanoparticles had maximum absorption at 400-415 nm, an average size of 20-25 nm and a spherical and uniform shape. Antimicrobial activity of showed that the antimicrobial effects were concentration-dependent and in equal concentrations, the nanoparticles had a higher inhibitory effect than aquetic extract, in which at 50µ g/mL the highest inhibitory effect was observed against Staphylococcus aureus (19 mm) and Aspergillus flavus (19 mm). Based on the results, plants have a high potential for biosynthesis of nanoparticles that can be used as antiseptic in various industries.

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