In order to investigate and compare the effect of external application of hormonal treatments on some biochemical characteristics and one-thousand grain weight in three wheat cultivars in terms of degree of tolerance to water deficit period including Pishtaz (tolerant), Sabalan (semi-tolerant) and Gaspard (susceptible) under drought tension during onset booting origin, the pot experiment as factorial design with four replications was conducted in Yazd University in 2015. Treatments included cultivar factor at three levels, drought tension at two levels (100% field capacity or control, 50% or drought tension) and hormone application at four levels without hormone, Kinetin (100 microMolar concentration), 24-epibrasinolide (1 microMolar) and their interaction. All analyzes were performed on the flag leaf. Drought incidence resulted in the most significant decrease in chlorophyll a, b, a + b content, chlorophyll a/b ratio, carotenoid, protein and one-thousand grain weight in susceptible cultivar (Gaspard). The highest significant increase in abscisic acid, soluble sugars, proline and soluble sugars / starch ratio was observed in tolerant cultivar. Among the hormonal treatments, 24epibrasinolide had the most significant increase in intrinsic abscisic acid and soluble sugars / starch ratios, and the interaction of Kinetin and 24-epibrasinolide had the highest increase in pigment content, proline and protein content, as well as one-thousand grain weight during drought incidence particularly in tolerant cultivar (Pishtaz). The results of the present research showed that the effect of hormonal treatments on biochemical indices was more than their effect on one-thousand grain weight. Overall, the interaction treatment of Kinetin and 24-epibrasinolide had more effect on biochemical indices and one-thousand grain weight than individual hormone treatments. Pishtaz (tolerant) cultivar has benefited more from hormone treatments to better withstand the dehydration period.