Lab-Scale Production of Biologically Active Form of Immunotoxin Targeted against Granulocyte Colony Stimulating Factor

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Ghodrati Siahmazgi m.; Nasiri Khalili m.a.; ZEINODDINI M.; KHODADADI S.; Zarkar s.; FARAMARZI N.

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Journal: JOURNAL OF CELL & TISSUE Year:2019 | Volume:10 | Issue:3 Page(s): 152-160

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Information Journal Paper

Title

Lab-Scale Production of Biologically Active Form of Immunotoxin Targeted against Granulocyte Colony Stimulating Factor

Author(s)

Keywords

ImmunotoxinQ2

Granulocyte Colony Stimulating FactorQ1

ExpressionQ1

purificationQ2

active formQ1

Abstract

Aim: This study aimed to produce recombinant hybrid protein, DT389GCSF, in E. coli BL-21(DE3) in a soluble and active form and to purify it and evaluate its cytotoxicity. Material and Methods: The protein, His6-tagged DT389GCSF was expressed in E. coli BL-21(DE3) in a soluble form at 28 ° C. Then it was purified by affinity chromatography on nickel sepharose column. Finally, the function of purified recombinant protein was evaluated by MTT assay on HL-60 cancer cell line. Results: The yield of Expression and purification of DT389GCSF were determined at about 12 and 80 percent, respectively, using Image J software. The IC50 value upon 48 hours of exposure of DT389GCSF toward cell line was about 0. 00017± 0. 000019 M. Conclusion: By reducing the temperature and using the intracellular mechanism, the refolding of hybrid protein, DT389GCSF, can be observed in a proper and active form. As a result, in vitro production of relevant Immunotoxins, by using this method, the steps of inclusion body production can be neglected.

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APA: Copy

Ghodrati Siahmazgi, m., Nasiri Khalili, m.a., ZEINODDINI, M., KHODADADI, S., Zarkar, s., & FARAMARZI, N.. (2019). Lab-Scale Production of Biologically Active Form of Immunotoxin Targeted against Granulocyte Colony Stimulating Factor. JOURNAL OF CELL & TISSUE, 10(3 ), 152-160. SID. https://sid.ir/paper/360435/en

Vancouver: Copy

Ghodrati Siahmazgi m., Nasiri Khalili m.a., ZEINODDINI M., KHODADADI S., Zarkar s., FARAMARZI N.. Lab-Scale Production of Biologically Active Form of Immunotoxin Targeted against Granulocyte Colony Stimulating Factor. JOURNAL OF CELL & TISSUE[Internet]. 2019;10(3 ):152-160. Available from: https://sid.ir/paper/360435/en

IEEE: Copy

m. Ghodrati Siahmazgi, m.a. Nasiri Khalili, M. ZEINODDINI, S. KHODADADI, s. Zarkar, and N. FARAMARZI, “Lab-Scale Production of Biologically Active Form of Immunotoxin Targeted against Granulocyte Colony Stimulating Factor,” JOURNAL OF CELL & TISSUE, vol. 10, no. 3 , pp. 152–160, 2019, [Online]. Available: https://sid.ir/paper/360435/en

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