MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES
Year:2013 | Volume:35 | Issue:2|Papers Count:19

Backgrounds and Objectives: Propolis is a resinous substance collected by honeybees from buds and leaves of trees and plants; it then mixed with pollen and secreted enzymes by bees. This study was aimed to assessing the anti-fungal activity of Ethanol Extract of Propolis in an experimental of cutaneous candidial infection.Materials and Methods: In this study, twenty-four male rabbits were studied. Animals were randomly divided into six equal groups: positive control group, negative control group, and four treated groups. The four test groups were which given different doses of EEP (125, 250, 500 and 1000 mg/ml) the positive control group received the standard Nystatin treatment and negative control group received only Topical Normal saline, in each groups local treatment was performed, two times a day for 20 days.Results: The results showed that EEP in its highest dose of 1000mg/ml was effective after 3 days of treatment in comparison. Clinical signs of cutaneous candidiasis were healed in Nystatin group after 5 days.Conclusion: Ethanol Extract of Propolis in concentration 1000mg/ml is an effective treatment for Candida albicans cutaneous candidiasis. It could be a suitable alternate for renowned chemical drugs.

Backgrounds and Objectives: Laryngeal cancer is one of the most common cancers of head and neck and its metastases to the lymph nodes are the main problem in the management of disease either for surgeon or in patient for its beauty problem.Materials and Methods: The purpose of this study was to evaluate the rate of neck lymph nodes metastasis in patients with laryngeal cancers based on tumor histopathology. In a descriptive-analytical study that performed on 200 patients with laryngeal cancer, rate of neck lymph nodes’ metastasis was evaluated and necessary information such as histopathology of laryngeal tumor was collected from patients’ records. Data were analyzed by statistical methods.Results: From total number of 200 patients, 197 were male, with the total mean age of 56.53±7.89 years. Seventy eight of patients had neck metastasis. Two patients had insitu squamous cell calcinoma (SCC) without neck metastases, 117 patients had well differentiated SCC with 10.3% neck metastases, 55 patients had moderately differented. SCC with 74.5% neck metastases, 19 patients had poorly diff. SCC with 011 % neck metastases, 6 patients had undifferentied. SCC with 100% neck metastases and one patient had Verocos carcinoma without neck metastases.Conclusion: Neck lymph nodes metastases in patients with laryngeal cancer is a common findingthat rates of it dependents on the site, size and tumors histopathology and degree of differentiation.

Backgrounds and Objectives: Toxoplasmosis is a parasitic disease and its main way of transmission is placenta to fetus pathway. If this transmission occurs in the 3th month of pregnancy consequences such as abortion, central nerve system abnormality and ocular disorder may happen. Because of this issue, the precise detection of Toxoplasma Antibodies such as as IgG and IgM has utmost importance that contains ELISA & ELISA AVIDITY.Materials and Methods: In this survey the serum and amniotic fluid samples that were collected from 48 pregnant women were collected and measurements were performed by ELISA AVIDITY kit.Results: The results of this study showed that the ELISA AVIDITY techniques able to detect the toxoplasma infection and its temporal occurrence.Conclusion: In ELISA technique the only antibody that could be detected precisely is IgM; but in this new technique IgG antibody also is detected, and enable us to better estimate the transmission time during the pregnancy.

seem that Ethnic and geographic factors are important elements. Cancer associated with mutation in tumor suppressor genes and oncogene. In addition to mutation, genetic polymorphisms are involved in incidence of cancer. The purpose of this investigation was studying polymorphism at codon 72 of p53 gene in patients with thyroid cancer in East Azerbaijan province.Material and Methods: In a case-control study 60 patients with thyroid cancer and 99 healthy individuals were studied as controls. After DNAs extraction from peripheral blood cells different genotypes of the 72th codon of p53 gene were determined by amplification refractory mutation system (ARMS) and presence of probable mutation, single strand conformation polymorphism (SSCP) technique was also conducted to confirm the results; Exon 4 of p53 gene was investigated by DNA sequencing.Results: In the control group, the genotype distribution of p53 polymorphism for Arg/Arg, Arg/Pro and Pro/Pro genotypes was 30.3%, 50.5% and 19.2% respectively. In the caner groups, the distribution was 31.67%, 51.67%, 16.67%, respectively for the same order of genotypes.Conclusion: This study indicates that polymorphism at codon 72 p53 gene is not a genetic predisposing factor for thyroid cancer in this population.

Backgrounds and Objectives: Heavy metals are toxic for human and animals and plants. For example nickel can cause contact dermatitis and may have carcinogenic effect. There are physicochemical and biological methods for removal of these elements from the environment. Some bacterial species are able to remove the elements by bioremediation mechanism. These types of bacteria could resist heavy metals and grow in the presence of these elements. In this research, the resistance of the nature of a heavy metal resistance bacteria’s that is bacteria’s living in Aran-Bidgol Lake has been evaluated.Materials and Methods: Seven Bacterial samples of the Lake were transferred on ventosa culture medium. Isolated colonies were grown on the media containing nickel transferred to mediums with other heavy metal elements. Biochemical and morphologic and also phylogenies studies based on sequencing of the 16S rDNA gene were performed in order to characterization genus and species of resistant isolates. Enzyme production of these bacteria’s was also investigated.Results: From 46 isolated halophilic bacteria, one isolate that was able to grow in the medium containing 1-25% NaCl and also was resistant to nickel and other toxic metal was considered for future study and characterization by sequencing of the 16S rDNA. This isolate had more 97% homology with Halomonas elongate species.Conclusion: Microorganism those are isolated from Aran-Bidgol Lake and are resistant to toxic metals have the potential usage for removal of environment toxins such as Nickel.

Background and Objectives: Interleukin-18 (IL-18) plays a key role in autoimmunity, inflammation and infection diseases and its higher levels are reported during the subclinical stage of type 1 diabetes mellitus. The aim of this study was to evaluate the recently described single-nucleotide-polymorphisms of promoter of IL-18 gene at position-137, in type 1 diabetic patients, which has been suggested to cause differences in transcription factor binding and IL-18 gene activity.Material and Methods: In this study, 104 type 1 diabetic patients (33female and 71 male) with mean age of 19.6±10.4 years, and 92 ages and sex matched, healthy individuals without family history of diabetes and autoimmune diseases were selected as controls. The single- nucleotide-polymorphism (SNP) at position-137 (G/C) in the promoter region of IL-18 gene was analyzed by sequence-specific PCR.Results: The genotype distribution differed significantly between patient and controls (p=0.038). The difference reflected as an increase in The GG genotypes at position-137 in promoter of IL-18 gene.Conclusion: The results of these studies suggest an association between type 1 diabetes and polymorphisms in the promoter region of IL-18.

Background & Objectives: Stroke incidence is a multifactorial phenomenon and investigation of the relationship between seasonal-regional variation and stroke is the focus of current studies. Based on seasonal, diurnal and hourly variations, incidence of different stroke was compared in this study.Material & Methods: In this retrospective study, 1817 medical records of stroke patients admitted in 2010 were reviewed from Ahvaz medi-educational hospitals. The type of aphasia was diagnosed according to the patients’ speech and language characteristics. SPSS-16 was used to analyze the data.Results: 39.9% of the patients had stroke-induced aphasia, and the highest rate of stroke incidence was found to be in the Summer time (38.2%) on Saturdays (16.1%), and between 8-12 A.M. (P<0.05).Conclusion: It seems that incidence of all kinds of stroke is influenced by time variation, and future works are needed to strengthen the results of our study.

Backgrounds and Objectives: House dust mites and cockroaches are important indoor allergen sources causing allergic rhinitis and asthma. The aim of this study was to investigate the house dust mite and cockroach prevalence using skin prick test (SPT).Materials and Methods: In this study 407 allergic rhinitis and asthmatic patients were evaluated. All patients were tested with the aeroallergens using house dust mix, Dermatophagoides farinae, Dermatophagoides pteronyssinus and cockroach mix. Data were analyses by Statistical analysis.Results: Total number of 407 patients 212 (52%) males and 195(48%) females, age between 4-70 years, were studied. In asthmatic and allergic rhinitis, the respective positive percentages for house dust, Dermatophagoides pteronyssinus, Dermatophagoides farinae and cockroaches were 23.3%, 31.7%, 35.6% and 32.9% respectively. Total IgE level elevated in over half of allergic patients.Conclusion: A relatively high prevalence of mites and cockroach sensitivity was found in our study. These figures are is similar with other reports from hot and humid areas.

Background and Objectives: Pneumonia severity assessment systems such as the pneumonia severity index (PSI) have been designed to help physicians to decide properly regarding the appropriate site of care in these patients. Along with such systems, serum inflammatory factors such as C-reactive protein (CRP) could be used for predicting the prognosis of patients with pneumonia. This study aimed to evaluate the correlation between on-admission quantitative CRP (qCRP) and PSI in hospitalized patients with pneumonia.Materials & Methods: During descriptive-analytical study, 60 hospitalized patients with pneumonia were evaluated in a 12-month period. On admission, risk stratification was performed based on the PSI system, and serum qCRP was measured. The correlation between on-admission qCRP and PSI was calculated. Mean values of qCRP was also compared between the expired and discharged patients.Results: 34 males (56.7%) and 26 females (43.3%) with a mean age of 65.03±17.47 years werestudied. Mean values of on-admission PSI and serum qCRP were 103.95±37.04 (range: 35-221) and 104.12±43.50 (range: 21-217) mg/L respectively. There was a significant positive correlation between on-admission serum qCRP and PSI(r=0.59, p<0.001). Mean serum qCRP was significantly higher in those who died compared with those who discharged (128.70±25.35 vs. 95.18±45.45 mg/L; p=0.01).Conclusion: Based on the results of current study, on-admission serum qCRP is well-correlated with PSI in hospitalized patients with pneumonia and, it can be used as a predicting marker of short-term prognosis.

Background and Objectives: The aim of the present study was to comparing attentional biases among the Patients with panic disorder, obsessive-compulsive disorder and normal individuals.Materials and Methods: For this purpose, 32 patient with panic disorder and obsessive-compulsive disorder and 32 normal individuals were selected accessibility. The groups were matched in their demographic characteristics with other. The subjects completed modified stroop task. The collected data was analyzed by descriptive statistic method of Multivariate Analysis of Variance (MANOVA).Results: The results showed differences between panic disorder patients and normal individuals and also between Obsessive-Compulsive Disorder and normal individuals. Also results showed that attentional bias in patients is with specific stimulus the disorder.Conclusion: Attentional bias toward the stimulus that are associated with panic disorders and obsessive compulsive disorder play a vital role in onset and maintenance of these disorders.

Background and objectives: Bronchiectasis is a late complication of mustard gas exposure with disturbing symptoms in its victims. There are some reports of successful treatment with prolonged effect of the symptoms by local steroid installation. In this study we evaluated the efficacy of local steroid installation in a group of musterd gas victims during the IRacl war.Material and method: This was an interventional pre-post treatment exposure study. We evaluated the effects of local steroids in 10 bronchiectasis patients by installation of steroid via bronchoscope. We measured lung volumes and airway resistance before procedure and again two weeks and three months after treatment implementation. Then we compared the results of three consecutive measurements. Friedman analysis was used for statistical analysis.Results: There was no important improvement in airway resistance; lung volumes and airflows after procedure, although treatment of bronchiectasis with local installation of steroids had no important complications. Of spirometric indices, only SReff was followed the substantial and significant increase.Conclusion: Local steroids are not effective treatment for mustard gas induce bronchiectasis.

Background and Objectives: Asbestos fibers are used extensively in various industries including brake and clutch linings and airborne asbestos emission in urban environments can induce serious effects on human health, including pulmonary fibrosis and mesothelioma. The aim of this study was to access asbestos fibers emission in high traffic area of Tabriz city.Materials and Methods: In this study, that was down 2012 (between ……. a ……) the concentration of asbestos fibers was measured at 12 high traffic areas of Tabriz city. The air samples were collected on membrane filters using integrated environmental sampling method for identification of asbestos fibers phase-contrast optical microscope was used to count the fibers with a length to width ratio greater than three.Results: The average concentration of asbestos fibers in high traffic areas (0.001±0.0003 fiber/ml of air) was far more than the standard limits (0.00005 fiber/ ml of air). Also, the results of this study showed that the highest concentration of asbestos fibers was found in downtown area of Baghshmal square (0.0004± 0.0012 fiber/ml of air) and the lowest concentration was found in Shariati square (0.00056 ±0.00012 fiber/ml of air).Conclusion: This study showed that emissions of asbestos fibers from urban sources like such as vehicle brakes is so high that could create, increased respiratory complications and obligates us for more preventive measures.

Background and Objective: Environmental and lifestyle factors are believed to play a large role in the incidence of colorectal cancer which is currently the third most common cancer in the world. In Iran, colorectal cancer has markedly increased over the last several decades. The objective of the present study was to determine epidemiological factors associated and with increased risk of cancer among the Iranian population.Materials and Methods: In this retrospective study that was done in radiotherapy department of Tabriz University of Medical Sciences. The association between risk factors such as life style, familial history, diabetes and hypertension were studied in 100 consequative patients who were referred to radiotherapy department. The information was processed by statistical methods.Results: This studied population consisted of 30 women and 70 men, mean age of them was 49.3±5.1 years. Low physical activity, low fiber diet, Obesity and smoking were found among 96.9%, 62.2%, 41% and 42% of them respectively diabetes, hypertension, and dyslipidemia were also found among 27%, 37% and 27% of these.Conclusion: Dietary fiber has long been thought to be associated with a reduced risk of rectal cancer. Low physical activity and metabolic syndrome including’s obesity, diabetes, hypertension and dyslipidemia are also associated with increased risk of rectal cancer.

Background & Objectives: This study was done to investigate the effect of lovastatin therapy on paraoxonase 1 (PON1) and arylesterase (ARE) activities, and low-density lipoprotein cholesterol (LDL-C) susceptibility to oxidation in people with type 2 diabetic nephropathy (T2DN).& Methods: Lovastatin (20 mg/day) was administered to 30 people with T2DN for 90 days and then withdrawn for 30 days. PON1 and ARE activities were measured by the spectrophotometric method. Susceptibility of LDL-C to oxidation was determined as the production of conjugated dienes.Results: After 90 days of lovastatin intervention, PON1 and ARE activities and LDL-C lag phase were significantly increased (p=0.004, 0.002, and b0.001). After 30 days of lovastatin withdrawal, PON1 and ARE activities and LDL-C lag phase had not changed significantly.Conclusion: Lovastatin therapy improves PON1 and ARE activities, and LDL-C susceptibility to oxidation. Despite withdrawal of lovastatin, PON1 and ARE activities, and LDL-C susceptibility to oxidation remain unchanged for a long period of time in people with T2DN.

Backgrounds and Objectives: A few researches have performed about the circadian rhythm of oxidants. The aim of this study was to investigate effect of aerobic exhaustive exercise on Circadian rhythm of MDA and PC in a group of healthy active collegiate men with normal night sleep.Materials and Methods: In this study. For this reason, 10 active students with mean of age: 23.2±2.57 year's old, height: 174.5±6.35 cm and weight: 65.9±4.5 kg, voluntarily participated in this study. All participants followed recommended sleep- wake programs and diet regime during two weeks for synchronization. Their wake-sleep and diet programs were under supervision from the night of beginning of sampling up to 48 hours. Control and experimental, with one week interval between them. In control group, six blood samples were collected at 4 hourly intervals started 12:00 (mid-day) until the upcoming morning samples were collected from antecubital vein. In experimental group, similar protocol was implemented. Colorimetric chemical method was used for measuring MDA and PC factors. Repeated Measures ANOVA was used for statistical study.Results: The results showed that the plasma MDA levels exhibited a highly significant circadian rhythm in control (P=0.014) and experimental (p=0.008) groups. But, There weren’t a significant circadian rhythm for PC factor (P<0.05).Conclusion: The result of this study showed that there is a high risk of oxidative stress injuries in the morning and exercise can vary affect rhythm of oxidative stress.

ARC syndrome is an abbreviation of arthrogryposis, renal and cholestasis. It is a rare syndrome but needs awareness of physicians. During past 4 years two cases with direct hyperbilirubinemia, polyuria, ichtiosis and skeletal malformation have been diagnosed, both of them were term born from consanguine parents, and present their symptom and signs during the first month of birth. Their liver transaminase was mildly evaluated and one of them had proximal renal tubular acidosis and another one had combined proximal and distal renal tubular acidosis.ARC presents itself by prolonged neonatal icter, unilateral or bilateral arthrogryposis, and variable renal findings but polyuria, polydypsia and acidosis are constant renal findings of ARC.