Background and Aims: The current research aimed to examine the effectiveness of Acceptance and Commitment Therapy (ACT) on educational vitality and family functioning of students with Physical-motor injuries. Materials and Methods: The study employed a pretest-posttest quasi-experimental design. The sample consisted of 30 students with physical-motor injuries and their mothers who were selected through purposeful sampling. The participants were randomly divided into experiment and control groups and completed individual profile form as well as family functioning, and educational vitality questionnaires. The experiment group received 10 twohour sessions of ACT. Finally, the collected data were analyzed using the covariance analysis statistical method. Results: The results indicated a significant difference between pretest and posttest as well as pretest and follow-up phases in terms of family functioning and educational vitality scores between the witness and experimental groups (P<0. 05). Conclusion: Considering the findings of the present study showing the effectiveness of ACT on the family functioning and educational vitality, this method can be implemented to improve these variables in the students with physical-motor injuries and their mothers.