Background and aim: The number and configuration of root canals in maxillary molars particularly in mesiobuccal roots display a wide range of variations. Therefore, enough knowledge of root canals anatomy, prior to root canal therapy, is an important factor for a successful treatment and long-term prognosis. Root canal anatomies have been studied by different techniques. The purpose of this study was to determine the percentage of root canal types in maxillary molars in Iranian population using staining and clearing techniques.Materials and Methods: In this study, 100 human first and second maxillary molars (50 of each) were studied to determine the type and number of root canals and also the presence of accessory and lateral canals. Root canal systems were studied with a little modification using staining and clearing technique based on Vertucci"s classification. Results: Different types of canals in mesiobuccal root of first maxillary molars were as follows: 20% type 1, 32% type 2, 40% type 4, 4% type 5, 4% type 6, in 30% accessory canals and in 20% lateral canals were seen, in distobuccal root 90% typel, 10% type 5 in 22% accessory canals and in 14% lateral canals were seen, in palatal root 92% type 1, 8% type 5 and in 30% accessory canals and in 18% lateral canals were seen.Different types of canals in mesiobuccal root of second maxillary molars were as follows: 74% type 1, 14% type 2, 10% type 4, 2% type 5 and in 32% accessory canals and in 8% lateral canals were seen, in distobuccal root 94% type 1, 6% type 5 and in 20% accessory canals and in 8% lateral canals were seen, and in palatal root 100% type 1, 30% accessory canals and 12% lateral canals were seen. First and second maxillary molars had two canals in the mesiobuccal root as 76% and 24%, respectively. The percentage of type 1 canals in distobuccal and palatal roots of both molars were similar and more than other types.Conclusion: Because of high prevalence of different types of canals in mesiobuccal root of maxillary molars, careful exploration for extra canals during access cavity preparation is mandatory, otherwise treatment failure is inevitable