Iron deficiency that usually appears as chlorosis in young leaves, is considered to be one of the most important nutritional disorders in citrus trees, particularly in calcareous soils, that seriously reduces yield and fruits quality. Selecting the resistant rootstocks to iron chlorosis is one of the most important approaches to prevent this nutritional problem. Therefore, an experiment was carried out at Jahrom Agricultural Research Station, the effects of resistant rootstocks like Macrophylla (Citrus macrophylla Wester), Volkamer lemon (Citrus volkameriana), and Sour orange (Citrus autantium) in comparison with prevailing Sour lime (Citrus aurantifolia Swing) rootstock and also their interactions with scions like ‘Valencia’ orange, ‘Washington Navel’ orange, Jahrom local orange and red pulp orange (Moro) were investigated. Also the effects of sequestrene 138 Fe in three levels 0 (control), 5 and 10 gram per tree were compared. The Macrophylla and Sour orange rootstocks had the best positive and significant effects in increasing the scions leaf chlorophyll index at 1% statistical level. Mocrophylla also had the highest iron absorption. The interaction between rootstocks and sequestrene 138 Fe showed that the application of sequestrene 138 Fe levels on Macrophylla rootstock did not have a significant effect on leaf chlorophyll index at 1% statistical level, therefore Macrophylla had high potential in increasing scions leaf chlorophyll index without any use of sequestrene 138 Fe as fertilizer. Sour orange had lower iron absorption than other rootstocks, but significantly increased scions leaf chlorophyll index. In contrast, Sour lime with high potential for iron absorption had little effect on scion leaf chlorophyll index. This, did not only clearly showed the effect of rootstocks on scions leaf iron chlorosis Paradox, but also indicated that the effectiveness of rootstock in resisting iron chlorosis is related to mechanisms other than the extent of iron absorption by the roots. Macrophylla significantly increased iron, zinc, manganese and copper absorption comoared to other rootstocks at 1% statistical level; moreover, it absorbed the highest rates of phosphorus and potassium and a consideralde rate of nitrogen. This high absorption potential of Macrophylla put it in the group of effective rootstocks for Jahrom. The results of the experiment also demonstrated the significant difference between scions on resistance against iron chlorosis, i.e., the navel and local orange had high chlorophyll index irrespective of the rootstock used. In contrast, the scions like red pulp and ‘Valencia’ orange had lower chlorophyll index and were affected by the rootstock more than others. For example in comparison with Sour lime, the Macrophylla rootstock increased red pulp orange (Moro) leaf chlorophyll index about 18 percent.