مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

218
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

0
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

The Effect of Valproic Acid Drug on the Expression of SOCS1, SOCS3, JAK1, JAK2, STAT3, STAT5A and STAT5B Genes in HepG2 Cell Line Liver Cancer

Pages

  336-348

Abstract

 Background & aim: Aberrant activation of various intracellular pathways is involved in differentiation, growth, Apoptosis and cell survival. Different known intracellular pathways such as JAK/STAT cause tumor genesis and cancer development. This pathway plays an important role in various cellular functions and is activated by cytokines. Suppressors of cytokine signaling (SOCSs) genes play a pivotal role in regulating the immune system. Therefore, the aim of the research was to determine the effect of Valproic acid drug on the expression of SOCS1, SOCS3, JAK1, JAK2, STAT3, STAT5A and STAT5B genes in HepG2 cell line Liver cancer. Methods: In the present experimental study conducted in 2019, HepG2 Liver cancer cells were purchased from Pasteur Institute and the overlapping of the cells reached about 80%. Using trypsin, the cells were collected and after washing, they were cultured in 96-well plates. After 24 hours of cell culture, the culture medium was drained and the medium containing Valproic acid drug with different concentrations (0, 1, 5, 7. 5, 10, 15, 20 and 25 μ, mol) was replaced (solvent only control group). They received the drug, DMSO). After 24 and 48 hours, the cells were washed with PBS solution. MTT technique was used to determine cell viability. To determine the amount of apoptotic cells and gene expression, the cells were treated with Valproic acid with a concentration of 6. 643 μ, mol for 24 hours and 5. 401 μ, mol for 48 hours, and after 24 and 48 hours, flow cytometry and real-time techniques were used for Determination of apoptotic cells and expression of SOCS1, SOCS3, JAK1, JAK2, STAT3, STAT5A and STAT5B genes were used. The collected data were analyzed using one-way analysis of variance and Turkey’, s test. Results: Valproic acid significantly increased the expression of SOCS1 and SOCS3 genes, decreased the expression of JAK1, JAK2, STAT3, STAT5A and STAT5B genes and inhibited cell growth. This combination significantly caused Apoptosis (p<0. 001). The percentage of apoptotic cells after 24 and 48 hours was 22. 38 and 50. 3, respectively. The maximum amount of Apoptosis was observed after 48 hours. Conclusion: Valproic acid can induce Apoptosis in HepG2 Liver cancer through JAK/STAT pathway. Valproic acid seems to exert its apoptotic effect by decreasing the expression of JAK1, JAK2, STAT3, STAT5B genes and increasing SOCS1 and SOCS3 genes.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    Sanai Jahormi, M., & Kavoosi, F.. (2022). The Effect of Valproic Acid Drug on the Expression of SOCS1, SOCS3, JAK1, JAK2, STAT3, STAT5A and STAT5B Genes in HepG2 Cell Line Liver Cancer. ARMAGHAN DANESH, 27(3 (152) ), 336-348. SID. https://sid.ir/paper/1008399/en

    Vancouver: Copy

    Sanai Jahormi M., Kavoosi F.. The Effect of Valproic Acid Drug on the Expression of SOCS1, SOCS3, JAK1, JAK2, STAT3, STAT5A and STAT5B Genes in HepG2 Cell Line Liver Cancer. ARMAGHAN DANESH[Internet]. 2022;27(3 (152) ):336-348. Available from: https://sid.ir/paper/1008399/en

    IEEE: Copy

    M. Sanai Jahormi, and F. Kavoosi, “The Effect of Valproic Acid Drug on the Expression of SOCS1, SOCS3, JAK1, JAK2, STAT3, STAT5A and STAT5B Genes in HepG2 Cell Line Liver Cancer,” ARMAGHAN DANESH, vol. 27, no. 3 (152) , pp. 336–348, 2022, [Online]. Available: https://sid.ir/paper/1008399/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button