Adjunct Autophagy Suppression May Be a Promising Way to Eliminate the Resistance of Cancer Cells to Treatment

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Mamizadeh Zoleikha; Kalani Mohamad Reza; PARSANIA MASOUD; SOLTAN DALLAL MOHAMMAD MEHDI; MORADI ABDOLVAHAB

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Journal Paper

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Journal: Archives of Medical Laboratory Sciences Year:2021 | Volume:7 | Issue:1 Page(s): 0-0

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Title

Adjunct Autophagy Suppression May Be a Promising Way to Eliminate the Resistance of Cancer Cells to Treatment

Author(s)

Keywords

Apoptosis

Autophagy

Colorectal Cancer

M-protein

Oncolytic Virus

VSV

Abstract

Background and Aim: Autophagy is a highly conserved mechanism in eukaryotic cells which removes the dysfunctional organelles from the cell. Autophagy has immense physiological and pathological roles in cells. There are a variety of reports showing the dual function of Autophagy as a tumor suppression and promotion phenomenon. Therefore, targeted therapy approaches like virotherapy can be a promising cancer treatment. Vesicular stomatitis virus (VSV) is a well-known Oncolytic Virus which mutations in its matrix (M) protein including M51R, make it a better candidate for oncotargeting. Moreover, beclin-1 is one of the key regulators of Autophagy and also Apoptosis. Methods: In the present study, the level of Autophagy markers such as beclin-1 and LC3 were investigated concerning the Apoptosis process induction by VSV M-protein. Two Colorectal Cancer cell lines HCT116 and SW480 and one normal colon epithelial cell line (FHC) which expressing VSV M51R mutant M-protein were compared regarding Autophagy versus Apoptosis. All experiments were conducted at least in triplicate. Results: The results showed that the elevated level of caspase 3 and reduced amount of beclin-1 in transfected SW480 cells may be an acceptable description for Apoptosis. Conclusion: In HCT116 cells domination of Autophagy, plays a supportive mechanism for the cells to survive in response to M51R M-protein stress. Suppression of Autophagy as an adjunct can be a promising way to eliminate resistance to cancer treatment. We have quantitively evaluated the Beclin-1 and LC3-II as Autophagy markers, Future evaluation of these two along with other markers like P62 will reduce the limitations of this study.

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APA: Copy

Mamizadeh, Zoleikha, Kalani, Mohamad Reza, PARSANIA, MASOUD, SOLTAN DALLAL, MOHAMMAD MEHDI, & MORADI, ABDOLVAHAB. (2021). Adjunct Autophagy Suppression May Be a Promising Way to Eliminate the Resistance of Cancer Cells to Treatment. ARCHIVES OF MEDICAL LABORATORY SCIENCES, 7(1), 0-0. SID. https://sid.ir/paper/1020184/en

Vancouver: Copy

Mamizadeh Zoleikha, Kalani Mohamad Reza, PARSANIA MASOUD, SOLTAN DALLAL MOHAMMAD MEHDI, MORADI ABDOLVAHAB. Adjunct Autophagy Suppression May Be a Promising Way to Eliminate the Resistance of Cancer Cells to Treatment. ARCHIVES OF MEDICAL LABORATORY SCIENCES[Internet]. 2021;7(1):0-0. Available from: https://sid.ir/paper/1020184/en

IEEE: Copy

Zoleikha Mamizadeh, Mohamad Reza Kalani, MASOUD PARSANIA, MOHAMMAD MEHDI SOLTAN DALLAL, and ABDOLVAHAB MORADI, “Adjunct Autophagy Suppression May Be a Promising Way to Eliminate the Resistance of Cancer Cells to Treatment,” ARCHIVES OF MEDICAL LABORATORY SCIENCES, vol. 7, no. 1, pp. 0–0, 2021, [Online]. Available: https://sid.ir/paper/1020184/en

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