Evaluation of the gene expression level of 15-Lipoxygenase-1 and its metabolite (15-S-HETE) in patients with Multiple Sclerosis and its association with serum lipid profile

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Safizadeh Banafsheh; HOSHYAR REYHANE; MEHRPOUR MASOUD; BIJARI BITA; SHEIKHI ALIREZA; TAVAKOLI YARAKI MASOUMEH

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Journal: Razi Journal of Medical Sciences Year:2017 | Volume:24 | Issue:162 Page(s): 54-64

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Title

Evaluation of the gene expression level of 15-Lipoxygenase-1 and its metabolite (15-S-HETE) in patients with Multiple Sclerosis and its association with serum lipid profile

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Keywords

InflammationQ2

HPLCQ3

Gene expressionQ2

15-lipoxygenase enzymeQ1

Multiple sclerosisQ2

Abstract

Background: Multiple sclerosis is one of the most common autoimmune inflammatory diseases in young ages. Many aspects of this disease are still unknown. The aim of the present study was to investigate the expression and activity level of 15-lipoxygenase-1 (a producer of lipid peroxide and regulating Inflammation and immune responses) in peripheral blood mononuclear cells in patients with Multiple sclerosis and healthy individual and considering its correlation with lipid profile of individuals. Methods: 30 patients with Multiple sclerosis from Firouzgar Hospital in Tehran and 23 healthy subjects as control participated in this case-control study. The peripheral blood mononuclear cells of the subjects were used for mRNA extraction and cDNA construction, and to determine the expression level of 15-Lipoxygenase-1 gene, a Real-Time PCR-based Cyber Green method was used and data were analyzed using Δ Δ CT method. Also, the amount of 15-lipoxygenase-1 product (15-S-HETE) in serum was determined by High Pressure Liquid Chromatography (HPLC). Finally, statistical analysis was performed using Graph Pad Prism software version 5 and independent t-test. Results: Measurement of 15-lipoxygenase-1 expression level in mononuclear blood cells extracted from the peripheral blood of patients with Multiple sclerosis and healthy controls revealed that the level of this gene was significantly increased in patients comparing to controls. Also, the activity of 15-lipoxygenase-1 which was measured via its metabolite level in serum of patients and controls demonstrated that the enzyme activity was increased in serum of patients compared to controls (p<0. 05). Also, the results have shown that in patients with higher level of Cholesterol, Triglyceride and LDL, the level of 15-lipoxygensae-1 and its metabolite had higher level compared to patients with normal lipid profile. Conclusion: The results of the current study have shown that the 15-lipoxygenase-1 enzymatic pathway might affect Multiple sclerosis pathogenesis and due to the significant differences of the enzyme level in patients compared to controls, it can be noticed as a possible pathway for controlling disease. Since elevated lipid profile level is the risk factor of Multiple sclerosis pathogenesis, in this study the correlation was observed between the higher level of LDL, Cholesterol and Triglyceride and enzyme expression level and activity which emphasize on the important role of lipid metabolic pathway in Multiple sclerosis.

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APA: Copy

Safizadeh, Banafsheh, HOSHYAR, REYHANE, MEHRPOUR, MASOUD, BIJARI, BITA, SHEIKHI, ALIREZA, & TAVAKOLI YARAKI, MASOUMEH. (2017). Evaluation of the gene expression level of 15-Lipoxygenase-1 and its metabolite (15-S-HETE) in patients with Multiple Sclerosis and its association with serum lipid profile. RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES), 24(162 ), 54-64. SID. https://sid.ir/paper/10341/en

Vancouver: Copy

Safizadeh Banafsheh, HOSHYAR REYHANE, MEHRPOUR MASOUD, BIJARI BITA, SHEIKHI ALIREZA, TAVAKOLI YARAKI MASOUMEH. Evaluation of the gene expression level of 15-Lipoxygenase-1 and its metabolite (15-S-HETE) in patients with Multiple Sclerosis and its association with serum lipid profile. RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES)[Internet]. 2017;24(162 ):54-64. Available from: https://sid.ir/paper/10341/en

IEEE: Copy

Banafsheh Safizadeh, REYHANE HOSHYAR, MASOUD MEHRPOUR, BITA BIJARI, ALIREZA SHEIKHI, and MASOUMEH TAVAKOLI YARAKI, “Evaluation of the gene expression level of 15-Lipoxygenase-1 and its metabolite (15-S-HETE) in patients with Multiple Sclerosis and its association with serum lipid profile,” RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES), vol. 24, no. 162 , pp. 54–64, 2017, [Online]. Available: https://sid.ir/paper/10341/en

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