Anticancer Effect of Fluorouracil and Gum-Based Cerium Oxide Nanoparticles on Human Malignant Colon Carcinoma Cell Line (Caco2 )

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KERAMATI ZAHRA; MOTALLEB GHOLAMREZA; RAHDAR ABBAS; KERACHIAN MOHAMMAD AMIN

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Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2023 | Volume:25 | Issue:3 Page(s): 194-202

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Information Journal Paper

Title

Anticancer Effect of Fluorouracil and Gum-Based Cerium Oxide Nanoparticles on Human Malignant Colon Carcinoma Cell Line (Caco2 )

Author(s)

KERAMATI ZAHRA | MOTALLEB GHOLAMREZA | RAHDAR ABBAS | KERACHIAN MOHAMMAD AMIN | Issue Writer Certificate

Keywords

Apoptosis

Caspases

Colorectal Neoplasms

Oncogenes

Abstract

Objective: We investigated whether co-incubation of 5-FU and gum-based cerium oxide nanoparticles (CeO2 NPs) would improve half-maximal inhibitory concentration (IC50 ) and Apoptosis in the Caco-2 cancer cell line Materials and Methods: In this experimental study, we synthesized Ceo-2-XG by the nano perception method. X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), dynamic light scattering (DLS) and vibrating sample magnetometer (VSM) techniques were employed to characterize the synthesized nanoparticles. The Caco-2 cancer cells were cultured and treated with Ceo-2XG and 5-FU. Cytotoxicity analysis was carried out using MTT assay on Caco-2 cancer cells. CXCR1, CXCR2, CXCL8, BAX, BCL-2, P53, CASPASE-3, CASPASE-8 and CASPASE-9 gene expression changes were assessed by quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR). The Caco-2 cancer cell mortality mechanism was analyzed using Annexin V-FITC/PI flow cytometry. Using the inverted microscope morphology changes of the Caco-2 cancer cells was observed. Results: With a sample size of roughly 11 nm, TEM analysis revealed spherical structures. Interestingly, after 72 hours, 400 μ, g/ml nanoparticles significantly lowered the IC50 of 5-FU from 101 to 71 μ, g/ml (P<000. 1). Furthermore, qRT-PCR analysis showed that BCL-2, CXCR1, CXCR2 and CXCR8 expressions were significantly decreased in the 5-FU and Ceo-2-XG nanoparticles co-incubated group, compared to the 5-FU alone (P<0. 001). Notably, gene expressions of BAX, P53, CASPASE-3, CASPASE-8 and CASPASE-9 were significantly higher in the 5-FU and Ceo2-XG nanoparticles co-incubated group, compared to the 5-FU alone (P<0. 001). The findings revealed that dead cells owing to Apoptosis were more than two times higher in 5-FU and Ceo-2-XG nanoparticles cancer cells than in 5-FU alone treated cancer cells. Conclusion: Co-incubation of 5-FU and Ceo-2-XG nanoparticles significantly increased Apoptosis in the Caco-2 cancer cells. The antiproliferative activity of co-incubated 5-FU and Ceo-2-XG nanoparticles on Caco-2 cancer cells was substantially higher than that of 5-FU alone.

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APA: Copy

KERAMATI, ZAHRA, MOTALLEB, GHOLAMREZA, RAHDAR, ABBAS, & KERACHIAN, MOHAMMAD AMIN. (2023). Anticancer Effect of Fluorouracil and Gum-Based Cerium Oxide Nanoparticles on Human Malignant Colon Carcinoma Cell Line (Caco2 ). CELL JOURNAL (YAKHTEH), 25(3), 194-202. SID. https://sid.ir/paper/1038192/en

Vancouver: Copy

KERAMATI ZAHRA, MOTALLEB GHOLAMREZA, RAHDAR ABBAS, KERACHIAN MOHAMMAD AMIN. Anticancer Effect of Fluorouracil and Gum-Based Cerium Oxide Nanoparticles on Human Malignant Colon Carcinoma Cell Line (Caco2 ). CELL JOURNAL (YAKHTEH)[Internet]. 2023;25(3):194-202. Available from: https://sid.ir/paper/1038192/en

IEEE: Copy

ZAHRA KERAMATI, GHOLAMREZA MOTALLEB, ABBAS RAHDAR, and MOHAMMAD AMIN KERACHIAN, “Anticancer Effect of Fluorouracil and Gum-Based Cerium Oxide Nanoparticles on Human Malignant Colon Carcinoma Cell Line (Caco2 ),” CELL JOURNAL (YAKHTEH), vol. 25, no. 3, pp. 194–202, 2023, [Online]. Available: https://sid.ir/paper/1038192/en

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