A STEREOLOGICAL STUDY OF KIDNEY VOLUME IN GLYCEROL-INDUCED ACUTE RENAL FAILURE IN MALE RATS

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DEHGHANI FARZANEH; DEZFOULIAN A.; PANJEH SHAHIN M.R.; TABEEI S.Z.A.D.; MOMBEYNI H.; ZAHIRI SH.

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Journal: JOURNAL OF MEDICAL RESEARCH (JMR) Year:2002 | Volume:1 | Issue:1 Page(s): 9-14

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Title

A STEREOLOGICAL STUDY OF KIDNEY VOLUME IN GLYCEROL-INDUCED ACUTE RENAL FAILURE IN MALE RATS

Author(s)

Keywords

STEREOLOGYQ3

RENAL FAILUREQ4

GLYCEROLQ3

Abstract

Background: Quantitative changes of histopathological structures are important in the process of improvement or regression of diseases. The aim of this study was to stereologically assess kidney volume (cortex and medulla) in GLYCEROL-induced acute RENAL FAILURE (ARF). Materials and Methods: Twenty-four Sprague Dawley rats were selected and randomly divided into two groups. In the case group, ARF was induced by intramuscular injection of 50% GLYCEROL (10 ml/kg). The control group received the same amount of saline solution. After 48 hours, anesthetized rats were sacrificed and the right kidneys were fixed by formalin perfusion. Kidneys were embedded in agar block and 1 mm thick slices were prepared by microtome. Each slice was processed and stained by hematoxylin eosin. Microscopic slides were used to measure the kidney volume using the point counting method and Cavalieri principle. Results: Kidney volume in the experimental group was more than that in the control group and there was a significant relationship between the cortex and whole kidney volumes. Conclusion: It seems that pathological changes coincided with volume changes. Although this procedure is unbiased, the results should be supported by further studies.

Cites

STEREOLOGICAL STUDY OF THE EFFECT OF NOISE STRESS ON THE FETUS MOUSE KIDNEY

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APA: Copy

DEHGHANI, FARZANEH, DEZFOULIAN, A., PANJEH SHAHIN, M.R., TABEEI, S.Z.A.D., MOMBEYNI, H., & ZAHIRI, SH.. (2002). A STEREOLOGICAL STUDY OF KIDNEY VOLUME IN GLYCEROL-INDUCED ACUTE RENAL FAILURE IN MALE RATS. JOURNAL OF MEDICAL RESEARCH (JMR), 1(1), 9-14. SID. https://sid.ir/paper/105858/en

Vancouver: Copy

DEHGHANI FARZANEH, DEZFOULIAN A., PANJEH SHAHIN M.R., TABEEI S.Z.A.D., MOMBEYNI H., ZAHIRI SH.. A STEREOLOGICAL STUDY OF KIDNEY VOLUME IN GLYCEROL-INDUCED ACUTE RENAL FAILURE IN MALE RATS. JOURNAL OF MEDICAL RESEARCH (JMR)[Internet]. 2002;1(1):9-14. Available from: https://sid.ir/paper/105858/en

IEEE: Copy

FARZANEH DEHGHANI, A. DEZFOULIAN, M.R. PANJEH SHAHIN, S.Z.A.D. TABEEI, H. MOMBEYNI, and SH. ZAHIRI, “A STEREOLOGICAL STUDY OF KIDNEY VOLUME IN GLYCEROL-INDUCED ACUTE RENAL FAILURE IN MALE RATS,” JOURNAL OF MEDICAL RESEARCH (JMR), vol. 1, no. 1, pp. 9–14, 2002, [Online]. Available: https://sid.ir/paper/105858/en

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