Evaluation of the Presence of Gene Encoding Loa22 in Pathogenic Leptospira Serovars

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Malek Mohammadi Yeganeh; KHAKI PEJVAK; MORADI BIDHENDI SOHEILA; Nofeli Mojtaba

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Journal Paper

Paper Information

Journal: Iranian Journal of Medical Microbiology Year:2022 | Volume:16 | Issue:5 Page(s): 392-398

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Information Journal Paper

Title

Evaluation of the Presence of Gene Encoding Loa22 in Pathogenic Leptospira Serovars

Author(s)

Malek Mohammadi Yeganeh | KHAKI PEJVAK | MORADI BIDHENDI SOHEILA | Nofeli Mojtaba | Issue Writer Certificate

Keywords

Leptospirosis

loa22 gene

PCR

Serovar

Abstract

Background and Aim: Leptospirosis is one of the most common zoonotic diseases worldwide, occurring mostly in tropical, subtropical, temperate, and humid regions with heavy rainfall. It is important to diagnose this condition correctly and promptly. Loa22 is an outer membrane protein exposed to the surface in some Leptospira Serovars. The purpose of this study was to determine the presence of the gene encoding Loa22 protein in Leptospira interrogans Serovars. Materials and Methods: The present study was conducted on 23 pathogenic leptospira Serovars and two non-pathogenic leptospira Serovars. These Serovars were prepared from the Reference Laboratory for Leptospira, Department of Microbiology, Razi Vaccine, and Serum Research Institute, Karaj, Iran. After genomic DNA extraction using the standard phenol-chloroform method, loa22 gene was amplified by specific primers. Results: PCR was performed on the loa22 gene by producing a 671-bp fragment. The results showed that the loa22 gene was present in all 23 pathogenic leptospira Serovars but not in the non-pathogenic L. biflexa. The specificity of tested primers was confirmed as well. Conclusion: The loa22 gene is a specific gene for pathogenic leptospira Serovars that is not found in saprophytic Serovars, so it is suggested that this gene be used to detect leptospira pathogenic Serovars.

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APA: Copy

Malek Mohammadi, Yeganeh, KHAKI, PEJVAK, MORADI BIDHENDI, SOHEILA, & Nofeli, Mojtaba. (2022). Evaluation of the Presence of Gene Encoding Loa22 in Pathogenic Leptospira Serovars. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, 16(5 ), 392-398. SID. https://sid.ir/paper/1062723/en

Vancouver: Copy

Malek Mohammadi Yeganeh, KHAKI PEJVAK, MORADI BIDHENDI SOHEILA, Nofeli Mojtaba. Evaluation of the Presence of Gene Encoding Loa22 in Pathogenic Leptospira Serovars. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY[Internet]. 2022;16(5 ):392-398. Available from: https://sid.ir/paper/1062723/en

IEEE: Copy

Yeganeh Malek Mohammadi, PEJVAK KHAKI, SOHEILA MORADI BIDHENDI, and Mojtaba Nofeli, “Evaluation of the Presence of Gene Encoding Loa22 in Pathogenic Leptospira Serovars,” IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, vol. 16, no. 5 , pp. 392–398, 2022, [Online]. Available: https://sid.ir/paper/1062723/en

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