Alpha-Amylase Immobilization: Methods and Challenges

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MAFAKHER LADAN; Ahmadi Yasin; KHALILI FARD JAVAD; YAZDANSETAD SAJJAD; Rezaei Gomari Sina; Elyasi Far Babak

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Journal Paper

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Journal: PHARMACEUTICAL SCIENCES Year:2023 | Volume:29 | Issue:2 Page(s): 144-155

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Title

Alpha-Amylase Immobilization: Methods and Challenges

Author(s)

Keywords

Alpha-amylase

Immobilization

Starch

Vmax

Abstract

Alpha-amylase is one of the most widely used enzymes in the Starch industry. However, industrial application of soluble Alpha-amylase is hampered by changes in pH and temperature (adverse effects on enzyme stability) and activity loss, leading to higher costs. Immobilization of alphaamylase is an efficient strategy to reduce the enzyme losing and subsequently reduces costs in this regard. Alpha-amylases are immobilized by adsorption, entrapment, covalent attachment, and cross-linking. A barrier in Alpha-amylase Immobilization is the large size of its substrate, namely amylose and amylopectin. Most of these Immobilization methods decrease the affinity of the enzyme for its substrate as well as the maximum rate of reaction (Vmax). This review aims to study different aspects of Alpha-amylase including enzyme activity, applications, structure, Starch, Immobilization methods, and Immobilization’, s obstacles to improve Alpha-amylase efficiency in the industry and also lowering the costs related to providing this enzyme.

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References

Alpha‐amylase as molecular target for treatment of diabetes mellitus: A comprehensive review

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APA: Copy

MAFAKHER, LADAN, Ahmadi, Yasin, KHALILI FARD, JAVAD, YAZDANSETAD, SAJJAD, Rezaei Gomari, Sina, & Elyasi Far, Babak. (2023). Alpha-Amylase Immobilization: Methods and Challenges. PHARMACEUTICAL SCIENCES, 29(2), 144-155. SID. https://sid.ir/paper/1064779/en

Vancouver: Copy

MAFAKHER LADAN, Ahmadi Yasin, KHALILI FARD JAVAD, YAZDANSETAD SAJJAD, Rezaei Gomari Sina, Elyasi Far Babak. Alpha-Amylase Immobilization: Methods and Challenges. PHARMACEUTICAL SCIENCES[Internet]. 2023;29(2):144-155. Available from: https://sid.ir/paper/1064779/en

IEEE: Copy

LADAN MAFAKHER, Yasin Ahmadi, JAVAD KHALILI FARD, SAJJAD YAZDANSETAD, Sina Rezaei Gomari, and Babak Elyasi Far, “Alpha-Amylase Immobilization: Methods and Challenges,” PHARMACEUTICAL SCIENCES, vol. 29, no. 2, pp. 144–155, 2023, [Online]. Available: https://sid.ir/paper/1064779/en

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