COMPARISON OF DERIVATION AND SUCCESSFUL PURIFICATION OF HUMAN ADIPOSE AND BONE MARROW-MESENCHYMAL STEM CELLS

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NADERI MESHKIN HOJJAT; MOGHADDAM MATIN MARYAM; AHMADIANKIA NAGHMEH; HEIRANI TABASI ASIEH; MIRAHMADI MAHDI; SHAHRYARI MINA; HASANZADEH MALIHEH; MAHMOUDI M.; SANJAR MOUSSAVI NASSER; ATTARANZADEH ARMIN; BAHRAMI AHMAD REZA

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Journal: Journal of Knowledge & Health in Basic Medical Sciences Year:2015 | Volume:9 | Issue:4 Page(s): 38-45

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Title

COMPARISON OF DERIVATION AND SUCCESSFUL PURIFICATION OF HUMAN ADIPOSE AND BONE MARROW-MESENCHYMAL STEM CELLS

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Keywords

MESENCHYMAL STEM CELLSQ2

ADIPOSE TISSUEQ2

BONE MARROWQ3

DERIVATION AND EXPANSIONQ3

Abstract

Introduction: MESENCHYMAL STEM CELLS (MSCs) exhibit multiple beneficial properties in treatment of various diseases through their differentiation capacity and secretion of paracrine signals. Therefore, using MSCs as a tool for cell therapy rely on the ability of harvesting a sufficient number of cells in a short time.Methods: In this study, MSCs derived from BONE MARROW (BM) and ADIPOSE TISSUE (AD) were investigated in terms of success rate of culture and cell yields. To ensure that the isolated cells are MSCs, adipogenic and osteogenic differentiation study were performed by Oil Red O, Alizarin Red S and Alkaline Phosphatase assay, and specific surface markers expression examined by flow cytometry.Results: The results showed that successful culture's rate of AD-MSCs were superior to BM-MSCs. AD-MSCs reached to confluency and passage in a shorter time (faster) and produced further amount of cells at a defined time. It was also demonstrated that the derivation of MSCs from ADIPOSE TISSUE were completely reproducible and not donor dependent rather than BM-MSCs.Conclusion: In conclusion, with our protocol, ADIPOSE TISSUEs are proposed the more appropriate source for derivation of MSCs in terms of percentage of cells successfully isolated and subsequent purification as compared to BONE MARROW.

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APA: Copy

NADERI MESHKIN, HOJJAT, MOGHADDAM MATIN, MARYAM, AHMADIANKIA, NAGHMEH, HEIRANI TABASI, ASIEH, MIRAHMADI, MAHDI, SHAHRYARI, MINA, HASANZADEH, MALIHEH, MAHMOUDI, M., SANJAR MOUSSAVI, NASSER, ATTARANZADEH, ARMIN, & BAHRAMI, AHMAD REZA. (2015). COMPARISON OF DERIVATION AND SUCCESSFUL PURIFICATION OF HUMAN ADIPOSE AND BONE MARROW-MESENCHYMAL STEM CELLS. KNOWLEDGE AND HEALTH, 9(4), 38-45. SID. https://sid.ir/paper/108063/en

Vancouver: Copy

NADERI MESHKIN HOJJAT, MOGHADDAM MATIN MARYAM, AHMADIANKIA NAGHMEH, HEIRANI TABASI ASIEH, MIRAHMADI MAHDI, SHAHRYARI MINA, HASANZADEH MALIHEH, MAHMOUDI M., SANJAR MOUSSAVI NASSER, ATTARANZADEH ARMIN, BAHRAMI AHMAD REZA. COMPARISON OF DERIVATION AND SUCCESSFUL PURIFICATION OF HUMAN ADIPOSE AND BONE MARROW-MESENCHYMAL STEM CELLS. KNOWLEDGE AND HEALTH[Internet]. 2015;9(4):38-45. Available from: https://sid.ir/paper/108063/en

IEEE: Copy

HOJJAT NADERI MESHKIN, MARYAM MOGHADDAM MATIN, NAGHMEH AHMADIANKIA, ASIEH HEIRANI TABASI, MAHDI MIRAHMADI, MINA SHAHRYARI, MALIHEH HASANZADEH, M. MAHMOUDI, NASSER SANJAR MOUSSAVI, ARMIN ATTARANZADEH, and AHMAD REZA BAHRAMI, “COMPARISON OF DERIVATION AND SUCCESSFUL PURIFICATION OF HUMAN ADIPOSE AND BONE MARROW-MESENCHYMAL STEM CELLS,” KNOWLEDGE AND HEALTH, vol. 9, no. 4, pp. 38–45, 2015, [Online]. Available: https://sid.ir/paper/108063/en

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