Melting Curve-based Assay as an Alternative Technique for the Accurate Detection of SARS-CoV-2

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Aboutalebian Shima; Mirzaaghaei Somayeh; Fakhim Hamed; Faramarzi Sama; MOUSAVI SOMAYEH; GHAFEL SAFIYEH; Gholipour sahar; FARHANG ARMIN; MIRHENDI HOSSEIN; NIKAEEN MAHNAZ

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Journal: Advanced Biomedical Research Year:2022 | Volume:11 | Issue:- Page(s): 0-0

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Title

Melting Curve-based Assay as an Alternative Technique for the Accurate Detection of SARS-CoV-2

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Abstract

Background: Early and cost‑, effective diagnosis and monitoring of the infection caused by severe acute respiratory syndrome coronavirus 2 (SARS‑, CoV‑, 2) are critically important to anticipate and control the disease. We aimed to set up a SYBR Green‑, based one‑, step real‑, time polymerase chain reaction (PCR) as a lower‑, cost alternative method to detect the virus. Materials and Methods: An in‑, house SYBR Green‑, based PCR assay targeting the envelope (E) and RNA‑, dependent RNA polymerase (RdRp) genes, was set up to diagnose the infection, and was compared with the reference probe‑, based PCR method. Results: When the commercial probe‑, based assay was considered as the reference method, SYBR Green‑, based PCR had a slightly lower sensitivity (81. 98% and 86. 25% for E and RdRp targets, respectively) and a good specificity (100% and 94. 44% for E and RdRp targets, respectively). For both gene targets, three different melting temperature (Tm) patterns were found in the PCRs of the nasopharyngeal/oropharyngeal swab samples, but no size polymorphism was seen in agarose gel electrophoresis. Conclusion: Further studies to improvement of the assay are needed to make it an inexpensive and reliable tool for the diagnosis of COVID‑, 19.

Cites

Conventional Reverse Transcription-Polymerase Chain Reaction Assay as an Alternative, Low-Cost, and Reliable Method for the Detection of Coronavirus Disease 2019 (Methods Article)

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APA: Copy

Aboutalebian, Shima, Mirzaaghaei, Somayeh, Fakhim, Hamed, Faramarzi, Sama, MOUSAVI, SOMAYEH, GHAFEL, SAFIYEH, Gholipour, sahar, FARHANG, ARMIN, MIRHENDI, HOSSEIN, & NIKAEEN, MAHNAZ. (2022). Melting Curve-based Assay as an Alternative Technique for the Accurate Detection of SARS-CoV-2. ADVANCED BIOMEDICAL RESEARCH, 11(-), 0-0. SID. https://sid.ir/paper/1083585/en

Vancouver: Copy

Aboutalebian Shima, Mirzaaghaei Somayeh, Fakhim Hamed, Faramarzi Sama, MOUSAVI SOMAYEH, GHAFEL SAFIYEH, Gholipour sahar, FARHANG ARMIN, MIRHENDI HOSSEIN, NIKAEEN MAHNAZ. Melting Curve-based Assay as an Alternative Technique for the Accurate Detection of SARS-CoV-2. ADVANCED BIOMEDICAL RESEARCH[Internet]. 2022;11(-):0-0. Available from: https://sid.ir/paper/1083585/en

IEEE: Copy

Shima Aboutalebian, Somayeh Mirzaaghaei, Hamed Fakhim, Sama Faramarzi, SOMAYEH MOUSAVI, SAFIYEH GHAFEL, sahar Gholipour, ARMIN FARHANG, HOSSEIN MIRHENDI, and MAHNAZ NIKAEEN, “Melting Curve-based Assay as an Alternative Technique for the Accurate Detection of SARS-CoV-2,” ADVANCED BIOMEDICAL RESEARCH, vol. 11, no. -, pp. 0–0, 2022, [Online]. Available: https://sid.ir/paper/1083585/en

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