Exosomes from adipose-derived stem cells promote angiogenesis and reduce necrotic grade in hindlimb ischemia mouse models

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Hoang Nhat Nguyen Trinh; Pham Phuc Van; Bich Vu Ngoc

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Journal Paper

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Journal: Iranian Journal of Basic Medical Sciences Year:2023 | Volume:26 | Issue:4 Page(s): 429-437

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Title

Exosomes from adipose-derived stem cells promote angiogenesis and reduce necrotic grade in hindlimb ischemia mouse models

Author(s)

Hoang Nhat Nguyen Trinh | Pham Phuc Van | Bich Vu Ngoc | Issue Writer Certificate

Keywords

Acute

Adipose-derived stem

cells Angiogenesis Exosome

Extracellular vesicles

Limb ischemia

Abstract

Objective(s): Acute hindLimb ischemia is a peripheral arterial disease that severely affects the patient’, s health. Injection of stem cells-derived exosomes that promote angiogenesis is a promising therapeutic strategy to increase perfusion and repair ischemic tissues. This study aimed to evaluate the efficacy of adipose stem cell-derived exosomes injection (ADSC-Exos) in treating Acute mouse hindLimb ischemia. Materials and Methods: ADSC-Exos were collected via ultracentrifugation. Exosome-specific markers were analyzed via flow cytometry. The morphology of exosomes was detected by TEM. A dose of 100 ug exosomes/100 ul PBS was locally injected into Acute mice ischemic hindlimb. The treatment efficacy was evaluated based on the oxygen saturation level, limb function, new blood vessel formation, muscle structure recovery, and limb necrosis grade. Results: ADSC-exosomes expressed high positivity for markers CD9 (76. 0%), CD63 (91. 2%), and CD81 (99. 6%), and have a cup shape. After being injected into the muscle, in the treatment group, many small and short blood vessels formed around the first ligation and grew down toward the second ligation. The SpO2 level, reperfusion, and recovery of the limb function are more positively improved in the treatment group. On day 28, the muscle’, s histological structure in the treatment group is similar to normal tissue. Approximately 33. 33% of the mice had grade I and II lesions and there were no grade III and IV observed in the treatment group. Meanwhile, in the placebo group, 60% had grade I to IV lesions. Conclusion: ADSC-Exos showed the ability to stimulate angiogenesis and significantly reduce the rate of limb necrosis.

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APA: Copy

Hoang Nhat Nguyen, Trinh, Pham, Phuc Van, & Bich Vu, Ngoc. (2023). Exosomes from adipose-derived stem cells promote angiogenesis and reduce necrotic grade in hindlimb ischemia mouse models. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 26(4), 429-437. SID. https://sid.ir/paper/1085297/en

Vancouver: Copy

Hoang Nhat Nguyen Trinh, Pham Phuc Van, Bich Vu Ngoc. Exosomes from adipose-derived stem cells promote angiogenesis and reduce necrotic grade in hindlimb ischemia mouse models. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2023;26(4):429-437. Available from: https://sid.ir/paper/1085297/en

IEEE: Copy

Trinh Hoang Nhat Nguyen, Phuc Van Pham, and Ngoc Bich Vu, “Exosomes from adipose-derived stem cells promote angiogenesis and reduce necrotic grade in hindlimb ischemia mouse models,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 26, no. 4, pp. 429–437, 2023, [Online]. Available: https://sid.ir/paper/1085297/en

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