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Title

Enhanced characterization of beta cell mass in a Tg(Pdx1-GFP) mouse model

Pages

  463-470

Abstract

 Introduction: Measurement of pancreatic beta cell mass in animal models is a common assay in diabetes researches. Novel whole-organ clearance methods in conjunction with transgenic mouse models hold tremendous promise to improve beta cell mass measurement methods. Here, we proposed a refined method to estimate the beta cell mass using a new transgenic Tg(Pdx1-GFP) mouse model and a recently developed free-of-acrylamide clearing tissue (FACT) protocol. Methods: First, we generated and evaluated a Tg(Pdx1-GFP) transgenic mouse model. Using the FACT protocol in our model, we could quantify the beta cell mass and alloxan-induced beta cell destruction in whole pancreas specimens. Results: Compiled fluorescent images of pancreas resulted in enhanced beta cell mass characterization in FACT-cleared sections (2928869±, 120215 AU) compared to No-FACT cleared sections (1292372±, 325632 AU). Additionally, the total number of detected islets with this method was significantly higher than the other clearance methods (155. 7 and 109, respectively). Using this method, we showed green fluorescent protein (GFP) expression confined to beta cells in Tg(Pdx1-GFP) transgenic. This enhanced GFP expression enabled us to accurately measure beta cell loss in a beta cell destruction model. The results suggest that our proposed method can be used as a simple, and rapid assay for beta cell mass measurement in islet biology and diabetes studies. Conclusion: The Tg(Pdx1-GFP) transgenic mouse in conjunction with the FACT protocol can enhance large-scale screening studies in the field of diabetes.

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    APA: Copy

    Asgari Abibeiglou, Behrouz, Pahlavanneshan, Saghar, FARROKHI, ALI, TAMADON, AMIN, BASIRI, MOHSEN, KHALOOGHI, KEYNOOSH, & FALLAHI, MAJID. (2022). Enhanced characterization of beta cell mass in a Tg(Pdx1-GFP) mouse model. BIOIMPACTS, 12(5), 463-470. SID. https://sid.ir/paper/1089747/en

    Vancouver: Copy

    Asgari Abibeiglou Behrouz, Pahlavanneshan Saghar, FARROKHI ALI, TAMADON AMIN, BASIRI MOHSEN, KHALOOGHI KEYNOOSH, FALLAHI MAJID. Enhanced characterization of beta cell mass in a Tg(Pdx1-GFP) mouse model. BIOIMPACTS[Internet]. 2022;12(5):463-470. Available from: https://sid.ir/paper/1089747/en

    IEEE: Copy

    Behrouz Asgari Abibeiglou, Saghar Pahlavanneshan, ALI FARROKHI, AMIN TAMADON, MOHSEN BASIRI, KEYNOOSH KHALOOGHI, and MAJID FALLAHI, “Enhanced characterization of beta cell mass in a Tg(Pdx1-GFP) mouse model,” BIOIMPACTS, vol. 12, no. 5, pp. 463–470, 2022, [Online]. Available: https://sid.ir/paper/1089747/en

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