The Effect of miR-4800 Restoration on Proliferation and Migration of Human Breast Cancer Cells In Vitro

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Khordadmehr Monireh; Matin Reyhaneh; BARADARAN BEHZAD; Baghbani Elham; Jigari Asl Farinaz; Noorolyai Saeed

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Journal: Advanced Pharmaceutical Bulletin Year:2023 | Volume:13 | Issue:2 Page(s): 378-384

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Title

The Effect of miR-4800 Restoration on Proliferation and Migration of Human Breast Cancer Cells In Vitro

Author(s)

Keywords

microRNA

Malignancy

Replacement

Therapeutic target

Abstract

Purpose: microRNAs (miRNAs) can contribute to cancer initiation, development, and progression. In this study, the effect of miRNA-4800 restoration on the growth and migration inhibition of human breast cancer (BC) cells was investigated. Methods: For this purpose, transfection of miR-4800 was performed into MDA-MB-231 BC cells using jetPEI. Subsequently, the expression levels of miR-4800 and CXCR4, ROCK1, CD44, and vimentin genes were measured using quantitative real-time polymerase chain reaction (q-RT-PCR) and specific primers. Also, the proliferation inhibition and apoptosis induction of cancer cells were evaluated by MTT and flow cytometry (Annexin V-PI method) techniques, respectively. Additionally, cancer cell migration after miR-4800 transfection was assessed by wound-healing (scratch) assay. Results: The restoration of miR-4800 in MDA-MB-231 cells resulted in the decreased expression level of CXCR4 (P ˂,0. 01), ROCK1 (P ˂,0. 0001), CD44 (P ˂,0. 0001), and vimentin (P ˂,0. 0001) genes. Also, MTT results showed restoration of miR-4800 could significantly reduce cell viability rate (P ˂,0. 0001) compared with the control group. Cell migration remarkably inhibited (P ˂,0. 001) upon miR-4800 transfection in treated BC cells. Flow cytometry data demonstrated that miR-4800 Replacement considerably induced apoptosis in cancer cells (P ˂,0. 001) compared with control cells. Conclusion: Taken together, it seems that miR-4800 can act as a tumor suppressor miRNA in BC and play an essential role in modulating apoptosis, migration, and metastasis in BC. Therefore, it may be suggested as a potential Therapeutic target in treating BC by performing additional tests in the future.

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APA: Copy

Khordadmehr, Monireh, Matin, Reyhaneh, BARADARAN, BEHZAD, Baghbani, Elham, Jigari Asl, Farinaz, & Noorolyai, Saeed. (2023). The Effect of miR-4800 Restoration on Proliferation and Migration of Human Breast Cancer Cells In Vitro. ADVANCED PHARMACEUTICAL BULLETIN, 13(2), 378-384. SID. https://sid.ir/paper/1090263/en

Vancouver: Copy

Khordadmehr Monireh, Matin Reyhaneh, BARADARAN BEHZAD, Baghbani Elham, Jigari Asl Farinaz, Noorolyai Saeed. The Effect of miR-4800 Restoration on Proliferation and Migration of Human Breast Cancer Cells In Vitro. ADVANCED PHARMACEUTICAL BULLETIN[Internet]. 2023;13(2):378-384. Available from: https://sid.ir/paper/1090263/en

IEEE: Copy

Monireh Khordadmehr, Reyhaneh Matin, BEHZAD BARADARAN, Elham Baghbani, Farinaz Jigari Asl, and Saeed Noorolyai, “The Effect of miR-4800 Restoration on Proliferation and Migration of Human Breast Cancer Cells In Vitro,” ADVANCED PHARMACEUTICAL BULLETIN, vol. 13, no. 2, pp. 378–384, 2023, [Online]. Available: https://sid.ir/paper/1090263/en

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