PRODUCTION OF CITRIC ACID FROM CHEMICALLY TREATED CANE MOLASSES BY IMMOBILIZED CELLS OF ASPERGILLUS NIGER

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VAHABZADEH F.; AMIRI S.

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Journal: AMIRKABIR Year:2001 | Volume:12 | Issue:46 Page(s): 163-176

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Title

PRODUCTION OF CITRIC ACID FROM CHEMICALLY TREATED CANE MOLASSES BY IMMOBILIZED CELLS OF ASPERGILLUS NIGER

Author(s)

VAHABZADEH F. | AMIRI S. | Issue Writer Certificate

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Abstract

Citric acid was produced from cane molasses treated chemically with tricalcium phosphate, by Aspergillus niger which was entrapped in calcium alginate beads. The citric acid fermentation was carried out in shake flasks and in order to get comparable results some other treatments were also conducted treated and untreated-canem olasses as the substrate for the fermentation and using A.niger in the form of free cells, untreated cane molasses and using A. niger in the form of immobilized cells. Time coursers) of three main operational variables in the citric acid fermentation was determined: citric acid produced, sugar utilized, sugar available (residual sugar) (both of these sugars were calculated as reducing sugar). Moreover, at the end of fermentation period (14 days) biomass yield was also determined (amount of biomass produced dry weigh based on amount of sugar utilized). With approach taken for comparing the trend of changes in concentration of the main fermentation parameters over time, results were discussed in detail and it was concluded that production of citric acid from treated cane molasses with using the immobilized cells was the most suitable conditions for the fermentation among other treatments. Citric acid concentration was 7.76 g / L (average value) and its yield based on amount of sugar utilized was 10.3%. In studying fermentation processes, it is desirable to use a mathematical model to present the kinetic behavior of product formation. Using such a mathematical formula to present the results could make some simplicity in studying the mechanism of the fermentation. In order to provide a rate expression for fermentation in most cases, however, one uses data from the plot of changes in substrate concentration versus time. In this research trend of decrease in substrate concentration over time (residual sugar in fermentation medium) showed to have a typical pattern, on the other hand changes in citric acid concentration during fermentation time had some fluctuation. The trend of increasing in sugar utilized during fermentation showed an exponential form and could be treated as first-order reaction. Rate of increasing in sugar utilization was taken as index for citric acid production along the biomass formation. First order rate constant was calculated as 3.452 × 10-3 hr-1.

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APA: Copy

VAHABZADEH, F., & AMIRI, S.. (2001). PRODUCTION OF CITRIC ACID FROM CHEMICALLY TREATED CANE MOLASSES BY IMMOBILIZED CELLS OF ASPERGILLUS NIGER. AMIRKABIR, 12(46), 163-176. SID. https://sid.ir/paper/1111/en

Vancouver: Copy

VAHABZADEH F., AMIRI S.. PRODUCTION OF CITRIC ACID FROM CHEMICALLY TREATED CANE MOLASSES BY IMMOBILIZED CELLS OF ASPERGILLUS NIGER. AMIRKABIR[Internet]. 2001;12(46):163-176. Available from: https://sid.ir/paper/1111/en

IEEE: Copy

F. VAHABZADEH, and S. AMIRI, “PRODUCTION OF CITRIC ACID FROM CHEMICALLY TREATED CANE MOLASSES BY IMMOBILIZED CELLS OF ASPERGILLUS NIGER,” AMIRKABIR, vol. 12, no. 46, pp. 163–176, 2001, [Online]. Available: https://sid.ir/paper/1111/en

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