OPTIMIZATION OF DISPERSIVE LIQUID-LIQUID FOR DETERMINATION OF TRACE SALIVARY MELATONIN USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

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POURHOSSEIN MEHRAN; SHAHTAHERI SEYED JAMALEDIN; MALECK KHANI HOMA; RAHIMI FOROUSHANI ABBAS; SEYED SOMEAH MIRGHANI; DIVANI RAZIEH

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Journal: IRAN OCCUPATIONAL HEALTH JOURNAL Year:2017 | Volume:14 | Issue:4 Page(s): 95-105

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Title

OPTIMIZATION OF DISPERSIVE LIQUID-LIQUID FOR DETERMINATION OF TRACE SALIVARY MELATONIN USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

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Keywords

MELATONINQ1

DISPERSIVE LIQUID–LIQUID MICROEXTRACTIONQ1

SALIVAQ1

CIRCADIAN RHYTHMQ1

HPLCQ1

Abstract

Background and aims: Due to low concentration of MELATONIN and also the existence of other compounds in body fluids, measuring the amount of MELATONIN is a serious challenge for the analysts. Before measurement of MELATONIN amounts using chromatographic method, sample preparation is unavoidable. The aim of current study is develop a new sample preparation method that not only have high accuracy and validity, but also can using by conventional equipment available in the laboratory to determine trace levels of SALIVAry MELATONIN. For this purpose, the dispersive liquid-liquid micro extraction (DLLME) method, that has not been used so far to preparation of SALIVAry MELATONIN samples, were optimized.Methods: Optimization procedure involved evaluation of eight factors affecting DLLME method. From these factors, seven were examined in four levels, and just pH was done in five levels. Accuracy and precision of the optimized method was evaluated at three concentrations of 50, 100, 250 pg.ml-1 by achieving CV% for day-to-day and within-day reproducibility.Results: Optimum values were determined. Accuracy and precision of the optimized method was investigated at three concentrations of 50, 100, and 250pg/ml by achieving CV% of 6.29, 2.39, and 1.82 for the "day-to-day" and 4.49, 2.68, and 1.83 for "within-day" reproducibility.Conclusion: Due to the results of this study for Coefficient of variations, the optimized method was provided with proper accuracy and precision. Although optimized extraction factors must be fit analyte, but these factors can be used for similar chemical compounds with a slight modification.

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APA: Copy

POURHOSSEIN, MEHRAN, SHAHTAHERI, SEYED JAMALEDIN, MALECK KHANI, HOMA, RAHIMI FOROUSHANI, ABBAS, SEYED SOMEAH, MIRGHANI, & DIVANI, RAZIEH. (2017). OPTIMIZATION OF DISPERSIVE LIQUID-LIQUID FOR DETERMINATION OF TRACE SALIVARY MELATONIN USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. IRAN OCCUPATIONAL HEALTH JOURNAL, 14(4 ), 95-105. SID. https://sid.ir/paper/128949/en

Vancouver: Copy

POURHOSSEIN MEHRAN, SHAHTAHERI SEYED JAMALEDIN, MALECK KHANI HOMA, RAHIMI FOROUSHANI ABBAS, SEYED SOMEAH MIRGHANI, DIVANI RAZIEH. OPTIMIZATION OF DISPERSIVE LIQUID-LIQUID FOR DETERMINATION OF TRACE SALIVARY MELATONIN USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. IRAN OCCUPATIONAL HEALTH JOURNAL[Internet]. 2017;14(4 ):95-105. Available from: https://sid.ir/paper/128949/en

IEEE: Copy

MEHRAN POURHOSSEIN, SEYED JAMALEDIN SHAHTAHERI, HOMA MALECK KHANI, ABBAS RAHIMI FOROUSHANI, MIRGHANI SEYED SOMEAH, and RAZIEH DIVANI, “OPTIMIZATION OF DISPERSIVE LIQUID-LIQUID FOR DETERMINATION OF TRACE SALIVARY MELATONIN USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY,” IRAN OCCUPATIONAL HEALTH JOURNAL, vol. 14, no. 4 , pp. 95–105, 2017, [Online]. Available: https://sid.ir/paper/128949/en

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