Development of dispersive liquid-liquid micro-extraction procedure for trace determination of pesticide diazinon in urine samples

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Ramin Sabet Maryam; POURHOSSEIN MEHRAN; KHADEM MONIREH; OMIDI FARIBORZ; GOLBABAEI FARIDEH; SHAHTAHERI SEYED JAMALEDDIN

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Journal: Journal of Health and Safety at Work Year:2019 | Volume:8 | Issue:4 Page(s): 359-370

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Title

Development of dispersive liquid-liquid micro-extraction procedure for trace determination of pesticide diazinon in urine samples

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Keywords

DiazinonQ2

Dispersive Liquid–Liquid Micro-ExtractionQ1

UrineQ1

HPLCQ1

Abstract

Introduction: Measurement of pesticides in biological matrices is become a serious challenge for researchers due to their very low concentration in different matrices. The aim of this study was to develop a new sample preparation method with high accuracy and validity and short retention time for determination of Diazinon. Material and Method: Dispersive liquid-liquid micro-extraction technique coupled with high performance liquid chromatography equipped with ultra violet detector was developed for trace extraction and determination of Diazinon pesticide in human Urine samples. One variable at a time method was used to optimize parameters affecting the Diazinon extraction. Different parameters such as extraction solvent, disperser solvent, and volume of the extraction solvent, volume of the disperser solvent, centrifugation time and speed, salt addition, and sample pH were studied and optimized. Result: Findings showed that optimal levels of these variables for Diazinon pesticide were 150 μ l of carbon tetrachloride as extraction solvent, 1. 5 ml of methanol as dispersive solvent, pH of 6, 5 minutes centrifuge time at speed of 4000 rpm, 0% (w/v) salt addition. Correlation coefficient was 0. 9965 indicating the linearity of a wide range of concentrations of the toxin. LOD and LOQ was calculated less than 0. 7 and 5 μ g L-1 respectively. The relative standard deviation for six replicate experiments in intra-day and inter-day at tree selected concentrations (50, 200 and 1000 μ g L-1) was less than 4% that indicates the accuracy and precision of the optimized method. Enrichment factor and extraction recovery for Diazinon were 245 and 99% respectively. Conclusion: According to the results, dispersive liquid-liquid micro-extraction procedure was successfully developed for the extraction of Diazinon from human Urine samples. Compared to other extraction techniques, the proposed procedure had some advantages such as shorter extraction time, better reproducibility, and higher enrichment factor.

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APA: Copy

Ramin Sabet, Maryam, POURHOSSEIN, MEHRAN, KHADEM, MONIREH, OMIDI, FARIBORZ, GOLBABAEI, FARIDEH, & SHAHTAHERI, SEYED JAMALEDDIN. (2019). Development of dispersive liquid-liquid micro-extraction procedure for trace determination of pesticide diazinon in urine samples. JOURNAL OF HEALTH AND SAFETY AT WORK, 8(4 ), 359-370. SID. https://sid.ir/paper/399741/en

Vancouver: Copy

Ramin Sabet Maryam, POURHOSSEIN MEHRAN, KHADEM MONIREH, OMIDI FARIBORZ, GOLBABAEI FARIDEH, SHAHTAHERI SEYED JAMALEDDIN. Development of dispersive liquid-liquid micro-extraction procedure for trace determination of pesticide diazinon in urine samples. JOURNAL OF HEALTH AND SAFETY AT WORK[Internet]. 2019;8(4 ):359-370. Available from: https://sid.ir/paper/399741/en

IEEE: Copy

Maryam Ramin Sabet, MEHRAN POURHOSSEIN, MONIREH KHADEM, FARIBORZ OMIDI, FARIDEH GOLBABAEI, and SEYED JAMALEDDIN SHAHTAHERI, “Development of dispersive liquid-liquid micro-extraction procedure for trace determination of pesticide diazinon in urine samples,” JOURNAL OF HEALTH AND SAFETY AT WORK, vol. 8, no. 4 , pp. 359–370, 2019, [Online]. Available: https://sid.ir/paper/399741/en

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