A MOLECULAR EPIDEMIOLOGY SURVEY OF CUTANEOUS LEISHMANIASIS IN PATIENT REFERRING TO PARASITOLOGY LAB AT SHIRAZ SCHOOL OF MEDICINE AND THE IMPORTANCE OF PCR ASSAY

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FAKHAR M.; MIKAEILI F.; HATAM G.R.; HABIBI P.; KARAMIAN M.; MOTAZEDIAN M.H.; BANIMOSTAFAVI E.

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Journal: Pars Journal of Medical Sciences Year:2010 | Volume:8 | Issue:1 Page(s): 1-6

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Title

A MOLECULAR EPIDEMIOLOGY SURVEY OF CUTANEOUS LEISHMANIASIS IN PATIENT REFERRING TO PARASITOLOGY LAB AT SHIRAZ SCHOOL OF MEDICINE AND THE IMPORTANCE OF PCR ASSAY

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Keywords

CUTANEOUS LEISHMANIASISQ3

MOLECULAR EPIDEMIOLOGYQ3

DEMOGRAPHICQ2

LEISHMANIA MAJORQ2

LEISHMANIA TROPICAQ2

Abstract

IntroductionFars Province is one of endemic foci of CUTANEOUS LEISHMANIASIS (CL). The present study aimed to conduct a MOLECULAR EPIDEMIOLOGY survey of patients referring to Parasitology Lab at Shiraz School of Medicine and evaluate the PCR assay for the diagnosis of CL.Material and MethodsThis retrospective study was carried out for patients referring to Parasitology department at Shiraz School of Medicine, during 1380-84. The disease was diagnosed by direct smear and staining with Giemsa; if the direct smear was negative, specific PCR was done on the DNA extracted from the direct smear to genus and species identification and culture. The data were registered and analyzed by SPSS software.ResultsOf 186 suspected patients, 104 (56%) cases were infected with CL. PCR results were positive in 35 (56.4%) out of 62 smears, among which microscopic examination did not reveal Leishmania amastigotes. Also, Leishmania species isolated from the patients residing in the urban and rural areas were LEISHMANIA TROPICA and LEISHMANIA MAJOR by species-specific primers, respectively.ConclusionSince in some cases the direct smear is reported negative, we suggest using PCR methods on smears in endemic regions.

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APA: Copy

FAKHAR, M., MIKAEILI, F., HATAM, G.R., HABIBI, P., KARAMIAN, M., MOTAZEDIAN, M.H., & BANIMOSTAFAVI, E.. (2010). A MOLECULAR EPIDEMIOLOGY SURVEY OF CUTANEOUS LEISHMANIASIS IN PATIENT REFERRING TO PARASITOLOGY LAB AT SHIRAZ SCHOOL OF MEDICINE AND THE IMPORTANCE OF PCR ASSAY. PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL), 8(1), 1-6. SID. https://sid.ir/paper/130954/en

Vancouver: Copy

FAKHAR M., MIKAEILI F., HATAM G.R., HABIBI P., KARAMIAN M., MOTAZEDIAN M.H., BANIMOSTAFAVI E.. A MOLECULAR EPIDEMIOLOGY SURVEY OF CUTANEOUS LEISHMANIASIS IN PATIENT REFERRING TO PARASITOLOGY LAB AT SHIRAZ SCHOOL OF MEDICINE AND THE IMPORTANCE OF PCR ASSAY. PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL)[Internet]. 2010;8(1):1-6. Available from: https://sid.ir/paper/130954/en

IEEE: Copy

M. FAKHAR, F. MIKAEILI, G.R. HATAM, P. HABIBI, M. KARAMIAN, M.H. MOTAZEDIAN, and E. BANIMOSTAFAVI, “A MOLECULAR EPIDEMIOLOGY SURVEY OF CUTANEOUS LEISHMANIASIS IN PATIENT REFERRING TO PARASITOLOGY LAB AT SHIRAZ SCHOOL OF MEDICINE AND THE IMPORTANCE OF PCR ASSAY,” PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL), vol. 8, no. 1, pp. 1–6, 2010, [Online]. Available: https://sid.ir/paper/130954/en

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