EVALUATION OF INHIBITORY AND LETHAL EFFECTS OF AQUEOUS, ETHANOLIC AND HYDROALCOHOLIC EXTRACTS OF AERIAL PARTS OF SALVIA CHORASSANICA AGAINST SOME GRAM-NEGATIVE AND GRAM-POSITIVE BACTERIA IN VITRO

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MEHRABAN AZAM; EDALATIAN DOVOM MOHAMMAD REZA; HADDAD KHODAPARAST MOHAMMAD HOSSEIN; MEHRABAN SANG ATASH MASOUMEH

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Journal: QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL Year:2016 | Volume:10 | Issue:2 Page(s): 1-11

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Title

EVALUATION OF INHIBITORY AND LETHAL EFFECTS OF AQUEOUS, ETHANOLIC AND HYDROALCOHOLIC EXTRACTS OF AERIAL PARTS OF SALVIA CHORASSANICA AGAINST SOME GRAM-NEGATIVE AND GRAM-POSITIVE BACTERIA IN VITRO

Author(s)

MEHRABAN AZAM | EDALATIAN DOVOM MOHAMMAD REZA | HADDAD KHODAPARAST MOHAMMAD HOSSEIN | MEHRABAN SANG ATASH MASOUMEH | Issue Writer Certificate

Keywords

ANTIQ1

BACTERIAL AGENTQ1

DISK DIFFUSION ANTIMICROBIAL TESTSQ1

KIRBYQ1

BAUER DISKQ1

DIFFUSION METHODQ1

AERIAL ORGANS OF SALVIACHORASSANICAQ1

SALVIA EXTRACTQ1

MICRODILUTIONQ1

Abstract

Background and Objectives: Development of bacterial resistance to ANTIbiotics has led to an increased tendency to development of new more effective and non-toxic ANTImicrobial compounds. In this study, the inhibitory and lethal effects of aqueous, ethanolic, and hydroalcoholic extracts of aerial parts of Salvia chorassanica were evaluated against Listeria monocytogenes, Bacillus cereus, Salmonella typhi, and Escherichia coli O: 157.Methods: In this study, KIRBY–Bauer disk DIFFUSION METHOD was used to evaluate ANTImicrobial activity. In this method, bacteria were cultivated as grass culture in Mueller Hinton Agar (MHA) media. To determine the minimum inhibitory concentration and minimum bactericidal concentration, micro-dilution method with ELISA and addition of phenyl tetrazolium chloride reagent, were used. Data were analyzed using one-way ANOVA and Duncan’s test at the significance level of p<0.05.Results: The highest diameter of inhibition in agar DIFFUSION METHOD was related to hydroalcoholic extract of aerial parts of Salvia chorassanica against Gram-positive bacteria Bacillus cereus. The amount of calculated MIC of hydro-alcoholic extract for Gram-positive bacteria was 30mg/ml. This amount was the lowest among other measured MIC.Conclusion: Based on the results of this study, Gram-negative bacteria showed more resistance to different extracts of aerial parts of Salvia chorassanica as compared to Gram-positive bacteria, so that Salmonella typhi was found to be the most resistant bacterium among the tested bacteria.

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APA: Copy

MEHRABAN, AZAM, EDALATIAN DOVOM, MOHAMMAD REZA, HADDAD KHODAPARAST, MOHAMMAD HOSSEIN, & MEHRABAN SANG ATASH, MASOUMEH. (2016). EVALUATION OF INHIBITORY AND LETHAL EFFECTS OF AQUEOUS, ETHANOLIC AND HYDROALCOHOLIC EXTRACTS OF AERIAL PARTS OF SALVIA CHORASSANICA AGAINST SOME GRAM-NEGATIVE AND GRAM-POSITIVE BACTERIA IN VITRO. QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL, 10(2), 1-11. SID. https://sid.ir/paper/131845/en

Vancouver: Copy

MEHRABAN AZAM, EDALATIAN DOVOM MOHAMMAD REZA, HADDAD KHODAPARAST MOHAMMAD HOSSEIN, MEHRABAN SANG ATASH MASOUMEH. EVALUATION OF INHIBITORY AND LETHAL EFFECTS OF AQUEOUS, ETHANOLIC AND HYDROALCOHOLIC EXTRACTS OF AERIAL PARTS OF SALVIA CHORASSANICA AGAINST SOME GRAM-NEGATIVE AND GRAM-POSITIVE BACTERIA IN VITRO. QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL[Internet]. 2016;10(2):1-11. Available from: https://sid.ir/paper/131845/en

IEEE: Copy

AZAM MEHRABAN, MOHAMMAD REZA EDALATIAN DOVOM, MOHAMMAD HOSSEIN HADDAD KHODAPARAST, and MASOUMEH MEHRABAN SANG ATASH, “EVALUATION OF INHIBITORY AND LETHAL EFFECTS OF AQUEOUS, ETHANOLIC AND HYDROALCOHOLIC EXTRACTS OF AERIAL PARTS OF SALVIA CHORASSANICA AGAINST SOME GRAM-NEGATIVE AND GRAM-POSITIVE BACTERIA IN VITRO,” QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL, vol. 10, no. 2, pp. 1–11, 2016, [Online]. Available: https://sid.ir/paper/131845/en

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