THE INHIBITORY EFFECT OF ARTEMISA QUTTENSIS EXTRACT ON NORA EFFLUX PUMP IN CIPROFLOXACIN RESISTANT STAPHYLOCOCCUS AUREUS STRAINS USING ETHIDIUM BROMIDE AND REAL-TIME PCR METHODS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MIRZAIE AMIR; BAGHERI KASHTALI ALIASGHAR; NOORBAZARGAN HASSAN; SADAT SHANDIZ SEYED ATAOLLAH

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL Year:2017 | Volume:11 | Issue:10 Page(s): 51-63

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

674

Download:

Cites:

Information Journal Paper

Title

THE INHIBITORY EFFECT OF ARTEMISA QUTTENSIS EXTRACT ON NORA EFFLUX PUMP IN CIPROFLOXACIN RESISTANT STAPHYLOCOCCUS AUREUS STRAINS USING ETHIDIUM BROMIDE AND REAL-TIME PCR METHODS

Author(s)

MIRZAIE AMIR | BAGHERI KASHTALI ALIASGHAR | NOORBAZARGAN HASSAN | SADAT SHANDIZ SEYED ATAOLLAH | Issue Writer Certificate

Keywords

ARTEMISIA QUTTENSISQ1

STAPHYLOCOCCUS AUREUSQ2

NORA EFFLUX PUMPQ1

Abstract

Background and Objectives: NORA EFFLUX PUMP is one of the mechanisms of ciprofloxacin resistance in STAPHYLOCOCCUS AUREUS strains, and finding natural herbal compounds with the ability to inhibit efflux pumps is one of the recent challenges. The aim of this study was to determine the inhibitory effect of Artemisa quttensis extract on NORA EFFLUX PUMP gene expression in ciprofloxacin resistantS. aureus strains.Methods: At first, A. quttensis ethanolic extract, was prepared by ethanol solvent. Then, existence of NORA EFFLUX PUMP was detected in 10 ciprofloxacin resistant clinical strains of S. aureus using polymerase chain reaction (PCR) method. Moreover, anti-efflux activity of the extract was determined using ethidium bromide method. Finally, after treatment of isolates with Sub-MIC concentration of the extract, norA gene expression level, was evaluated using real-time PCR method.Results: The PCR results showed that all of the strains had NORA EFFLUX PUMP, and and ethidium bromide method revealed that the A. quttensis extract had inhibitory effect on resistant strains that hadnorA efflux pump. Moreover, real-time PCR results showed that norA gene expression level was down-regulated by Sub-MIC concentrations of the extract in the ciprofloxacin resistant isolates.Conclusion: Considering the anti-efflux pump effect of A. quttensis extract, it seems that this extract has the potential to be used as a native plant in pharmaceutical industries.

Cites

No record.

References

No record.

Cite

APA: Copy

MIRZAIE, AMIR, BAGHERI KASHTALI, ALIASGHAR, NOORBAZARGAN, HASSAN, & SADAT SHANDIZ, SEYED ATAOLLAH. (2017). THE INHIBITORY EFFECT OF ARTEMISA QUTTENSIS EXTRACT ON NORA EFFLUX PUMP IN CIPROFLOXACIN RESISTANT STAPHYLOCOCCUS AUREUS STRAINS USING ETHIDIUM BROMIDE AND REAL-TIME PCR METHODS. QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL, 11(10 ), 51-63. SID. https://sid.ir/paper/131930/en

Vancouver: Copy

MIRZAIE AMIR, BAGHERI KASHTALI ALIASGHAR, NOORBAZARGAN HASSAN, SADAT SHANDIZ SEYED ATAOLLAH. THE INHIBITORY EFFECT OF ARTEMISA QUTTENSIS EXTRACT ON NORA EFFLUX PUMP IN CIPROFLOXACIN RESISTANT STAPHYLOCOCCUS AUREUS STRAINS USING ETHIDIUM BROMIDE AND REAL-TIME PCR METHODS. QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL[Internet]. 2017;11(10 ):51-63. Available from: https://sid.ir/paper/131930/en

IEEE: Copy

AMIR MIRZAIE, ALIASGHAR BAGHERI KASHTALI, HASSAN NOORBAZARGAN, and SEYED ATAOLLAH SADAT SHANDIZ, “THE INHIBITORY EFFECT OF ARTEMISA QUTTENSIS EXTRACT ON NORA EFFLUX PUMP IN CIPROFLOXACIN RESISTANT STAPHYLOCOCCUS AUREUS STRAINS USING ETHIDIUM BROMIDE AND REAL-TIME PCR METHODS,” QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL, vol. 11, no. 10 , pp. 51–63, 2017, [Online]. Available: https://sid.ir/paper/131930/en

Related Journal Papers