The Effect of Synthesis of Pegylated Nanoliposome Containing DNAi on Reduction of BCL-2 Gene Expression in DLCL2 Cell Line

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Boozari Saravani Farahnaz; TAFVIZI FARZANEH; JAAFARI MAHMOUD REZA

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL Year:2018 | Volume:12 | Issue:10 Page(s): 16-26

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

404

Download:

Cites:

Information Journal Paper

Title

The Effect of Synthesis of Pegylated Nanoliposome Containing DNAi on Reduction of BCL-2 Gene Expression in DLCL2 Cell Line

Author(s)

Boozari Saravani Farahnaz | TAFVIZI FARZANEH | JAAFARI MAHMOUD REZA | Issue Writer Certificate

Keywords

NanoQ1

LiposomesQ1

GenesQ1

bcl-2Q1

LymphomaQ1

Non-HodgkinQ1

Abstract

Background and Objectives: Non-Hodgkin Lymphoma is one of the common cancers that originates from lymphatic cells. The objective of this study was to assess the effectiveness of the DNAi containing pegylated Nanoliposome (antisense drug) on decrease of bcl-2 gene expression. Methods: In this study, to prepare DNAi containing pegylated Nanoliposome, 3 formulations were prepared with 5, 10, and 15% combinations of PEG 2000 with certain ratios of phospholipid and cholesterols. After purification by a Zetasizer, the mean diameter of Nanoliposome was measured. Drug efficacy was determined based on the standard curve of the drug. Cytotoxicity effect of the product was determined by treatment with 0. 2, 0. 5, and 0. 8μ g/μ l concentrations of each formulation in triplicate in vitro using MTT method. Then, bcl-2 gene expression was analyzed in DLCL2 cell line. Data were analyzed using one way ANOVA. Results: The mean diameter of the DNAi containing pegylated Nanoliposome was estimated to be 117 nm. The mean efficacy of the drug was 67%. The MTT assay on DLCL2 cell line indicated that the highest amount of cell growth inhibition was observed in treatment with pegylated NanoLiposomes 10% (PEG10) and the concentration of 0. 5μ g/μ l. Also, bcl-2 gene expression was significantly decreased in DLCL2 cell line in all concentrations of PEG10 in 48 hours, and the lowest level of bcl-2 gene expression was observed in 0. 5μ g/μ l concentration. Conclusion: PEG10 Nanoliposome formulation has higher efficacy in drug preservation and release compared to other two synthesized Liposomes. It seems that the synthesized Nanoliposome has the potential for using in pharmaceutical industries.

Cites

No record.

References

No record.

Cite

APA: Copy

Boozari Saravani, Farahnaz, TAFVIZI, FARZANEH, & JAAFARI, MAHMOUD REZA. (2018). The Effect of Synthesis of Pegylated Nanoliposome Containing DNAi on Reduction of BCL-2 Gene Expression in DLCL2 Cell Line. QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL, 12(10 ), 16-26. SID. https://sid.ir/paper/132065/en

Vancouver: Copy

Boozari Saravani Farahnaz, TAFVIZI FARZANEH, JAAFARI MAHMOUD REZA. The Effect of Synthesis of Pegylated Nanoliposome Containing DNAi on Reduction of BCL-2 Gene Expression in DLCL2 Cell Line. QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL[Internet]. 2018;12(10 ):16-26. Available from: https://sid.ir/paper/132065/en

IEEE: Copy

Farahnaz Boozari Saravani, FARZANEH TAFVIZI, and MAHMOUD REZA JAAFARI, “The Effect of Synthesis of Pegylated Nanoliposome Containing DNAi on Reduction of BCL-2 Gene Expression in DLCL2 Cell Line,” QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL, vol. 12, no. 10 , pp. 16–26, 2018, [Online]. Available: https://sid.ir/paper/132065/en

Related Journal Papers