The Effect of Progesterone on the Viability of MCF-7 Cell Line and Evaluation of Expression of P53, BAX and BCL-2 Genes

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ROSTAMI MOTAMED HADIS; SHARIATI MEHRDAD; AHMADI RAHIM; KHATAMSAZ SAEED; MOKHTARI MOKHTAR

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Journal: QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL Year:2019 | Volume:13 | Issue:7 Page(s): 1-9

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Title

The Effect of Progesterone on the Viability of MCF-7 Cell Line and Evaluation of Expression of P53, BAX and BCL-2 Genes

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Keywords

ProgesteroneQ3

ApoptosisQ1

MCF-7 cellQ2

Abstract

Background and Objectives: Researches have shown that Progesterone influences the viability of breast cancer cells. The purpose of this study was to investigate the effect of Progesterone on the viability of MCF-7 cell line and evaluation of Expression level of P53, Bax, and Bcl-2 Genes. Methods: In this laboratory-experimental study, MCF-7 cells were purchased from Pasture institute and divided into control group and the group received Progesterone in the concentrations of 0. 001, 0. 01, 0. 1, 1, and 10mg/mL. The viability of the cells was assessed using MTT assay. The relative expression level of P53, Bax, and Bcl-2 genes was investigated by Real Time PCR technique. Data analysis was performed using one-way ANOVA and independent samples t-test. Results: Exposure to 10mg/ml of Progesterone resulted in decreased viability of MCF-7 cells compared to the control group (p<0. 001). However, exposure to 0. 001, 0. 01, 0. 1, and 1mg/ml of Progesterone did not significantly alter the viability of the MCF-7 cells, as compared to the control group. The expression level of P53 and Bax genes significantly increased in MCF-7 cells exposed to the concentration 2. 5mg/ml of Progesterone compared to the control group (p<0. 05 and p<0. 001, respectively), but, the expression level of Bcl-2 gene significantly decreased compared to the control group (p<0. 001). Conclusion: The results of this study revealed that high concentration of Progesterone reduces the viability of MCF-7 cells and the cytotoxic effect of Progesterone on MCF-7 cells is performed by induction of Bax dependent Apoptosis and increased expression level of P53 tumor suppressor gene. Accordingly, Progesterone is important in the treatment of breast cancer.

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APA: Copy

ROSTAMI MOTAMED, HADIS, SHARIATI, MEHRDAD, AHMADI, RAHIM, KHATAMSAZ, SAEED, & MOKHTARI, MOKHTAR. (2019). The Effect of Progesterone on the Viability of MCF-7 Cell Line and Evaluation of Expression of P53, BAX and BCL-2 Genes. QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL, 13(7 ), 1-9. SID. https://sid.ir/paper/132106/en

Vancouver: Copy

ROSTAMI MOTAMED HADIS, SHARIATI MEHRDAD, AHMADI RAHIM, KHATAMSAZ SAEED, MOKHTARI MOKHTAR. The Effect of Progesterone on the Viability of MCF-7 Cell Line and Evaluation of Expression of P53, BAX and BCL-2 Genes. QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL[Internet]. 2019;13(7 ):1-9. Available from: https://sid.ir/paper/132106/en

IEEE: Copy

HADIS ROSTAMI MOTAMED, MEHRDAD SHARIATI, RAHIM AHMADI, SAEED KHATAMSAZ, and MOKHTAR MOKHTARI, “The Effect of Progesterone on the Viability of MCF-7 Cell Line and Evaluation of Expression of P53, BAX and BCL-2 Genes,” QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL, vol. 13, no. 7 , pp. 1–9, 2019, [Online]. Available: https://sid.ir/paper/132106/en

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