Investigation of Frequency of Mycoplasma hominis and Biological Parameters in Semen Sample of Men Referred to Qom Jihad Daneshgahi Infertility Treatment Center in 2016 (Iran)

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ASGARI ALI; NAZARI RAZIEH; Razavian Seyed Mohammad Hossein

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Journal: QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL Year:2018 | Volume:12 | Issue:4 Page(s): 81-88

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Title

Investigation of Frequency of Mycoplasma hominis and Biological Parameters in Semen Sample of Men Referred to Qom Jihad Daneshgahi Infertility Treatment Center in 2016 (Iran)

Author(s)

ASGARI ALI | NAZARI RAZIEH | Razavian Seyed Mohammad Hossein | Issue Writer Certificate

Keywords

Mycoplasma hominisQ1

InfertilityQ2

Polymerase chain reactionQ2

Abstract

Background and Objectives: Mycoplasmas hominis is one of the important causes of bacterial infections of the reproductive system, which through changes in the volume, pH, motility, morphology, and sperm count in men, can lead to reduction in the level of fertility or Infertility and sterility. In this research, the frequency of Mycoplasma hominis, was assessed by PCR method, and biological parameters, were investigated in the semen samples of infertile men referred to Qom jihad daneshgahi Infertility treatment center. Methods: In this descriptive-analytic study, a total of 187 semen samples were collected from infertile men referred to Qom jihad daneshgahi Infertility treatment center from April to September 2016. Semen analysis was performed according to the guideline of World Health Organization. After the extraction of genomic DNA, detection of Mycoplasma hominis, was performed by PCR method using 16S rRNA specific primers. Data were analyzed using dependent and independent t-test. Results: Among 187 semen samples studied by PCR, 71 samples (38%), were positive for Mycoplasma hominis. In the assessment of parameters, there was a significant association between bacterial infection and C class mean difference (in situ sperm motility( (p=0. 008). Conclusion: The results of the present study showed that high percentage of infertile men are infected with Mycoplasma hominis, which may lead to pelvic inflammatory diseases, urethritis, and Infertility. PCR method is a rapid and efficient technique for detection of mycoplasma, which can be useful in the control of sexual transmitted diseases.

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APA: Copy

ASGARI, ALI, NAZARI, RAZIEH, & Razavian, Seyed Mohammad Hossein. (2018). Investigation of Frequency of Mycoplasma hominis and Biological Parameters in Semen Sample of Men Referred to Qom Jihad Daneshgahi Infertility Treatment Center in 2016 (Iran). QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL, 12(4 ), 81-88. SID. https://sid.ir/paper/132274/en

Vancouver: Copy

ASGARI ALI, NAZARI RAZIEH, Razavian Seyed Mohammad Hossein. Investigation of Frequency of Mycoplasma hominis and Biological Parameters in Semen Sample of Men Referred to Qom Jihad Daneshgahi Infertility Treatment Center in 2016 (Iran). QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL[Internet]. 2018;12(4 ):81-88. Available from: https://sid.ir/paper/132274/en

IEEE: Copy

ALI ASGARI, RAZIEH NAZARI, and Seyed Mohammad Hossein Razavian, “Investigation of Frequency of Mycoplasma hominis and Biological Parameters in Semen Sample of Men Referred to Qom Jihad Daneshgahi Infertility Treatment Center in 2016 (Iran),” QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL, vol. 12, no. 4 , pp. 81–88, 2018, [Online]. Available: https://sid.ir/paper/132274/en

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