Assessment of Gold Nanoparticles Cytotoxicity Produced by the Biological Method by Fusarium Oxysporum on Normal and Cancerous Fibroblast Cell Lines

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Noorani Tahereh; Badiee Seyed Hossein; MANAFI SAHEBALI; Yahyaei Behrooz

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Journal: IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY Year:2018 | Volume:12 | Issue:1 Page(s): 23-32

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Title

Assessment of Gold Nanoparticles Cytotoxicity Produced by the Biological Method by Fusarium Oxysporum on Normal and Cancerous Fibroblast Cell Lines

Author(s)

Noorani Tahereh | Badiee Seyed Hossein | MANAFI SAHEBALI | Yahyaei Behrooz | Issue Writer Certificate

Keywords

Gold NanoparticlesQ1

Fusarium OxysporumQ1

CytotoxicQ1

Fibroblast Cell Lines CIRC-HLFQ1

Fibroblast Cell Lines SW 872Q1

Abstract

Background and Aims: Although there are several ways of nanoparticles production, however the biological method of nanoparticles production is under attention of researchers due to its eco friendly and energy saving properties. In the present study biosynthesis of gold nanoparticle was done by Fusarium Oxysporum fungus strain and the Cytotoxicity of the obtained nanoparticles was evaluated in the cell culture. Materials and Methods: Fusarium Oxysporum was purchased and cultured in Sabouraud dextrose broth medium. The culture supernatant was subjected to chloroauric acid solution at the final concentration of 1 mM. After nanoparticles production, the color changed reaction mixture was used for characterization with spectrophotometry, XRD and TEM microscopy. The obtained nanoparticles were washed and sterilized. Finally two cell lines (CIRC-HLF as normal and SW 872 as cancerous cell lines) were used for 3-(4, 5 dimethyl thiazol-2yl)-2, 5 diphenyl tetrazolium bromide (MTT) assay. Results: The fungal supernatant color after 24 hours was changed from yellow to red. TEM images have shown the nanoparticles were spherical or hexagonal and their sizes were around 50-70 nanometer. MTT assay and cell culture assay showed that the produced GNPs had toxic effect when they were used at high concentrations and the CIRC-HLF cell line was more resistant than SW 872 one against the higher concentration of GNPs. Conclusions: It seems that the biological production of Gold Nanoparticles by Fusarium Oxysporum can be done and can be easily produced, washed, sterilized and can be used in vivo.

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APA: Copy

Noorani, Tahereh, Badiee, Seyed Hossein, MANAFI, SAHEBALI, & Yahyaei, Behrooz. (2018). Assessment of Gold Nanoparticles Cytotoxicity Produced by the Biological Method by Fusarium Oxysporum on Normal and Cancerous Fibroblast Cell Lines. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, 12(1 ), 23-32. SID. https://sid.ir/paper/132587/en

Vancouver: Copy

Noorani Tahereh, Badiee Seyed Hossein, MANAFI SAHEBALI, Yahyaei Behrooz. Assessment of Gold Nanoparticles Cytotoxicity Produced by the Biological Method by Fusarium Oxysporum on Normal and Cancerous Fibroblast Cell Lines. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY[Internet]. 2018;12(1 ):23-32. Available from: https://sid.ir/paper/132587/en

IEEE: Copy

Tahereh Noorani, Seyed Hossein Badiee, SAHEBALI MANAFI, and Behrooz Yahyaei, “Assessment of Gold Nanoparticles Cytotoxicity Produced by the Biological Method by Fusarium Oxysporum on Normal and Cancerous Fibroblast Cell Lines,” IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, vol. 12, no. 1 , pp. 23–32, 2018, [Online]. Available: https://sid.ir/paper/132587/en

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