AN INVESTIGATION OF AAC(6’)-LE-APH(2

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SEYFI MAHNAZ; POURSHAFIEI M.R.; BORHANI K.; RAHIMI FATEH; SOLTAN DALAL MOHAMMAD MAHDI

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Journal: IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY Year:2007 | Volume:1 | Issue:1 Page(s): 33-38

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Title

AN INVESTIGATION OF AAC(6’)-LE-APH(2")-LA GENE IN MDR AND HLGR ENTEROCOCCUS FAECALIS AND E. FAECIUM STRAINS ISOLATED FROM CLINICAL SAMPLES

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ENTEROCOCCIQ4

HLGRQ4

MDRQ4

Abstract

Background: ENTEROCOCCI are important nosocomial pathogens. Multiple drug resistance (MDR) is common among ENTEROCOCCI and presents difficulties for treatment. High level gentamicin resistance (HLGR) in ENTEROCOCCI, is a significant therapeutic problem. Bactericidal antimicrobial activity usually is obtained by the synergistic combination of a cell wall active agent such as penicillin or glycopeptide with an aminoglycoside.ENTEROCOCCI can acquire aminoglycoside resistance genes that mediate production of aminoglycoside - modifying enzymes, which eliminate this synergistic bactericidal effect. The most clinically important HLGR genes is aac(6')-le-aph(2")-la.Material and methods: In the present study, a total of 437 clinical samples from 5 hospital and 3 private laboratory in Tehran , from azar 1384 to Tir 1385 , were collected and 300 enterococcal isolates recovered all of the strains were identified to the species level by conventional biochemical tests and assayed for their susceptibility to 11 antibiotics, ampicillin, tetracycline, erythromycin,ciprofloxacin, high dose gentamicin, vancomycin, cotrimoxazole, quinopristin -daifopristin (synercid), linezolid , teicoplanin and nitrofurantoin by disk diffusion method. Gentamicin MIC was accomplished for HLGR strains.Results: The most frequent species was E.faecalis (81.3%) and then E.faecium (18.7%). MDR strains were detected in 50% and 95% of Efaecalis and EfaeGium, respectively. The number of HLGR strains for Efaecalis and EfaeGium were found to be 19.5% and 23.5%, respectively. All HLGR strains showed MIC>1024mg/mL The PCR results showed that 83% and 100% of Efaecalis and EfaeGium strains carried aac(6')-le-aph(2")-la gene as detected by PCR.Conclusion: The present study indicates high rate dissemination ofaac(6')-le-aph(2")-la gene, suggesting the possible mechanism of transfer of gentamicin resistant genes within the enterococcal population and in this case probable need to new aminoglycosides or other antibiotics would be predictable.

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APA: Copy

SEYFI, MAHNAZ, POURSHAFIEI, M.R., BORHANI, K., RAHIMI, FATEH, & SOLTAN DALAL, MOHAMMAD MAHDI. (2007). AN INVESTIGATION OF AAC(6’)-LE-APH(2")-LA GENE IN MDR AND HLGR ENTEROCOCCUS FAECALIS AND E. FAECIUM STRAINS ISOLATED FROM CLINICAL SAMPLES. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, 1(1), 33-38. SID. https://sid.ir/paper/132619/en

Vancouver: Copy

SEYFI MAHNAZ, POURSHAFIEI M.R., BORHANI K., RAHIMI FATEH, SOLTAN DALAL MOHAMMAD MAHDI. AN INVESTIGATION OF AAC(6’)-LE-APH(2")-LA GENE IN MDR AND HLGR ENTEROCOCCUS FAECALIS AND E. FAECIUM STRAINS ISOLATED FROM CLINICAL SAMPLES. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY[Internet]. 2007;1(1):33-38. Available from: https://sid.ir/paper/132619/en

IEEE: Copy

MAHNAZ SEYFI, M.R. POURSHAFIEI, K. BORHANI, FATEH RAHIMI, and MOHAMMAD MAHDI SOLTAN DALAL, “AN INVESTIGATION OF AAC(6’)-LE-APH(2")-LA GENE IN MDR AND HLGR ENTEROCOCCUS FAECALIS AND E. FAECIUM STRAINS ISOLATED FROM CLINICAL SAMPLES,” IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, vol. 1, no. 1, pp. 33–38, 2007, [Online]. Available: https://sid.ir/paper/132619/en

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