ISOLATION OF LYTIC PHAGES AGAINST PATHOGENIC E.COLI ISOLATED FROM DIABTIC ULCERS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ZARE LEYLA; SHENAGARI MOHAMMAD; KHAN MIRZAEI MOHAMMADALI; MOJTAHEDI ALI

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY Year:2017 | Volume:11 | Issue:2 Page(s): 34-41

Download Full-Text

Persian Verion

View:

1,241

Download:

Cites:

Information Journal Paper

Title

ISOLATION OF LYTIC PHAGES AGAINST PATHOGENIC E.COLI ISOLATED FROM DIABTIC ULCERS

Author(s)

ZARE LEYLA | SHENAGARI MOHAMMAD | KHAN MIRZAEI MOHAMMADALI | MOJTAHEDI ALI | Issue Writer Certificate

Keywords

BACTERIOPHAGEQ2

ANTIBIOTIC RESISTANCEQ3

ESCHERICHIA COLIQ3

DIABETESQ2

WOUND INFECTIONQ2

Abstract

Background and Aims: In the present study, we have tried to isolate an effective lytic BACTERIOPHAGEs on drug-resistant ESCHERICHIA COLI isolated from diabetic ulcer wounds.Materials and Methods: Two E.coli strains were isolated by using standard methods from patients in Razi Hospital of Rasht. Waste water of the same hospital was used as a source for isolation of lytic BACTERIOPHAGEs. Agar layer method was used to isolate and plaque finding.Determination of host range of isolated phages was performed using five standard reference collection of E. coli. Finally, Transmission electron microscopy was used for morphological analysis of the isolated BACTERIOPHAGEs. Morphotype and family of isolated phages were determined based on Bradley recommendation and final version of International Committee on Taxonomy of Viruses (ICTV) report, respectively.Results: The results showed that the isolated bacteria were highly resistant to the antibiotics tested. Based on morphological characteristics, the separated virus belongs to siphoviridae family. Based on Bradley classification, isolated phage against strains of E.coli belongs to the morphotype B1.Conclusions: The isolated BACTERIOPHAGE from waste water efficiently lysed the multiresistant strains of E. coli. Therefore, BACTERIOPHAGEs could potentially be used as an alternative for antibiotics for treating infections by multiresistant E. coli.

Cites

In Vitro Biocontrol of Escherichia coli Through the Immobilization of its Specific Lytic Bacteriophage on Cellulose Acetate Biodegradable Film

References

Cite

APA: Copy

ZARE, LEYLA, SHENAGARI, MOHAMMAD, KHAN MIRZAEI, MOHAMMADALI, & MOJTAHEDI, ALI. (2017). ISOLATION OF LYTIC PHAGES AGAINST PATHOGENIC E.COLI ISOLATED FROM DIABTIC ULCERS. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, 11(2), 34-41. SID. https://sid.ir/paper/132627/en

Vancouver: Copy

ZARE LEYLA, SHENAGARI MOHAMMAD, KHAN MIRZAEI MOHAMMADALI, MOJTAHEDI ALI. ISOLATION OF LYTIC PHAGES AGAINST PATHOGENIC E.COLI ISOLATED FROM DIABTIC ULCERS. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY[Internet]. 2017;11(2):34-41. Available from: https://sid.ir/paper/132627/en

IEEE: Copy

LEYLA ZARE, MOHAMMAD SHENAGARI, MOHAMMADALI KHAN MIRZAEI, and ALI MOJTAHEDI, “ISOLATION OF LYTIC PHAGES AGAINST PATHOGENIC E.COLI ISOLATED FROM DIABTIC ULCERS,” IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, vol. 11, no. 2, pp. 34–41, 2017, [Online]. Available: https://sid.ir/paper/132627/en

Related Journal Papers