Expression Profile of Hyoscyamine Biosynthesis-related Genes in Response to UV-C Radiation in Datura metel Plant

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Karimian Ramin; Nafari Milad; Khodabakhshi Mohammad Reza; Davarpanah Seyed Javad

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Journal Paper

Paper Information

Journal: JOURNAL OF APPLIED BIOTECHNOLOGY REPORTS Year:621 | Volume: | Issue: Page(s): 1176-1181

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Title

Expression Profile of Hyoscyamine Biosynthesis-related Genes in Response to UV-C Radiation in Datura metel Plant

Author(s)

Karimian Ramin | Nafari Milad | Khodabakhshi Mohammad Reza | Davarpanah Seyed Javad | Issue Writer Certificate

Keywords

Datura metelQ4

UV-CQ4

H6HQ4

PMTQ4

TR-IQ4

HyoscyamineQ4

Abstract

Introduction: Tropane alkaloids as secondary metabolites are one of the most useful plant elements that are widely applied in medicinal approaches. Studies have shown that UV light led secondary metabolites to be increased. Thus, we investigated the effect of UV-C light on the expression of the main genes involved in the biosynthesis of tropane alkaloids, namely Hyoscyamine 6β-hydroxylase (H6H), Putrescine N-methyltransferase (PMT), and Tropinone reductase I (TR-I).Materials and Methods: Datura metel seeds cultured on MS media at 25 °C less than 12 h-12 h light-dark photoperiod. Then, they were transferred into vases and kept in the greenhouse. Three-month-old plants received 196 µWcm-2 UV-C light for 30 min. Afterward, the expression levels of different genes encoding H6H, PMT, and, TR-I enzymes, were measured at different post-exposure times.Results: Our results demonstrated that UV-C increased the expression of PMT and TR-I genes after 48 h. Moreover, the rise of H6H expression was found after 24 h but its level was downregulated again after 48 h.Conclusions: These findings indicated that UV-C light as abiotic stress could boost the formation of tropane alkaloids through upregulation of genes of enzymes catalyzing the main steps in their biosynthesis and, also, these genes are differentially affected.

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