IN VITRO INDUCTION OF FETAL HEMOGLOBIN IN ERYTHROID CELLS DERIVED FROM CD133+ CELLS BY TRANSFORMING GROWTH FACTOR- β AND STEM CELL FACTOR

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ATASHI AMIR; SOLEYMANI MASOUD; KAVIANI SAEID; HAJFATH ALI A.; AREFIAN E.

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Journal: Iranian Journal of Biotechnology Year:2008 | Volume:6 | Issue:3 Page(s): 157-163

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Title

IN VITRO INDUCTION OF FETAL HEMOGLOBIN IN ERYTHROID CELLS DERIVED FROM CD133+ CELLS BY TRANSFORMING GROWTH FACTOR- β AND STEM CELL FACTOR

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Keywords

CD133+ CELLSQ3

β-GLOBINQ3

FETAL HEMOGLOBINQ3

SCFQ3

TGF-YQ3

Abstract

Increased FETAL HEMOGLOBIN (HbF) in b-globin gene disorders ameliorates the clinical symptoms of the underlying disease. 5-azacytidine, butyrate and hydroxyurea, have been shown to activate g -globin gene expression. It has also been found that hematopoietic growth factors can influence expression of g-globin in erythroid cultures and in animal models. This study was designed to evaluate the in vitro effects of the stem cell factor (SCF) and transforming growth factor-b (TGF-b) on g-globin gene reactivation of erythroid precursors derived from CD133+ cells in vitro. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) analysis showed increased expression of the g-globin transcript in cell culture groups containing either TGF- b or SCF or both as compared to control (2.2-, 2.7- and 5.5-fold, respectively) (p<0.01). Production of HbF in a differentiated population was demonstrated using flow cytometry. The results of this study suggest that SCF and TGF-b warrant further evaluation as potential therapeutic drugs for the treatment of b-globin gene disorders.

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APA: Copy

ATASHI, AMIR, SOLEYMANI, MASOUD, KAVIANI, SAEID, HAJFATH ALI, A., & AREFIAN, E.. (2008). IN VITRO INDUCTION OF FETAL HEMOGLOBIN IN ERYTHROID CELLS DERIVED FROM CD133+ CELLS BY TRANSFORMING GROWTH FACTOR- β AND STEM CELL FACTOR. IRANIAN JOURNAL OF BIOTECHNOLOGY, 6(3), 157-163. SID. https://sid.ir/paper/139326/en

Vancouver: Copy

ATASHI AMIR, SOLEYMANI MASOUD, KAVIANI SAEID, HAJFATH ALI A., AREFIAN E.. IN VITRO INDUCTION OF FETAL HEMOGLOBIN IN ERYTHROID CELLS DERIVED FROM CD133+ CELLS BY TRANSFORMING GROWTH FACTOR- β AND STEM CELL FACTOR. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2008;6(3):157-163. Available from: https://sid.ir/paper/139326/en

IEEE: Copy

AMIR ATASHI, MASOUD SOLEYMANI, SAEID KAVIANI, A. HAJFATH ALI, and E. AREFIAN, “IN VITRO INDUCTION OF FETAL HEMOGLOBIN IN ERYTHROID CELLS DERIVED FROM CD133+ CELLS BY TRANSFORMING GROWTH FACTOR- β AND STEM CELL FACTOR,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 6, no. 3, pp. 157–163, 2008, [Online]. Available: https://sid.ir/paper/139326/en

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