ESTABLISHMENT, CULTURE AND FREEZING OF HUMAN AND MOUSE EMBRYONIC STEM CELLS: A PROTOCOL GUIDE

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BAHARVAND HOSSEIN; PAKZAD M.; TAEE A.; MOULA MOHAMMADI S.; REZAEI MEHRAN

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Journal: Anatomical Sciences Journal Year:2008 | Volume:5 | Issue:21-22 Page(s): 309-309

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Information Journal Paper

Title

ESTABLISHMENT, CULTURE AND FREEZING OF HUMAN AND MOUSE EMBRYONIC STEM CELLS: A PROTOCOL GUIDE

Author(s)

Keywords

FREEZINGQ3

EMBRYONIC STEM CELLSQ3

MOUSEQ3

HUMANQ3

Abstract

Studies of the biology of HUMAN EMBRYONIC STEM CELLS (hES cells) have developed rapidly over the past nine years since the first reports of their derivation. They clearly offer enormous potential, not only for regenerative medicine, but also for drug discovery and toxicology, HUMAN developmental biology and cancer research. Realizing these potentials a better understanding of the fundamental aspects of hES cells in vitro including maintaining , the quality of cells in their pluripotent state as well as improving aspects of experimental preparations , subpassaging and cryopereservation .The basic protocol describes the generation of MOUSE ES cells on MOUSE embryonic fibroblasts to provide simple method to hES cell establishment . Here we describe step by step the protocol of MOUSE and HUMAN ES cell generation, maintenance, subculturing , and cry preservation.

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