IN VITRO DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS INTO FUNCTIONAL NEURONS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

BAHARVAND H.; MATTHAEI K.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2003 | Volume:5 | Issue:19 Page(s): 155-163

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,722

Download:

Cites:

Information Journal Paper

Title

IN VITRO DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS INTO FUNCTIONAL NEURONS

Author(s)

BAHARVAND H. | MATTHAEI K. | Issue Writer Certificate

Keywords

EMBRYONIC STEM CELLSQ3

DIFFERENTIATIONQ2

NEURONQ3

Abstract

Introduction: Embryonic stem (ES) cells are pluripotent cells derived from inner cell mass of blastocyst. These cells can product any cell by spontaneous or directed DIFFERENTIATION. This study was started to produce NEURON cells by directed DIFFERENTIATION of new murine ES cell line in vitro. Materials and Methods: Murine ES cells (Royan B1 derived from C57BL/6 Strain) were used for in vitro NEURON DIFFERENTIATION. After production of embryoid bodies from ES cells, a highly enriched population of neuroepithelial precursor cells were derived from ES cell proliferation in the presence of growth factors (EGF and bFGF). These cells differentiate into both NEURONs and glia following withdrawal of growth factors and replacement of media. Immunohistochemistry and electrophysiology were used for evaluation of differentiated NEURONs. Results: Antibodies raised against microtubule associated proteins (MAP2), glutamic acid decarboxylase (GAD), and tyrosine hydroxylase (TH) antigens revealed NEURON cells with GABAergic and dopaminergic characteristics. Also, the NEURONs produced action potentials and responsed to electrical stimulations. Conclusion: Embryonic stem cell of Royan B1 can produce functional NEURON cells in vitro, by directed DIFFERENTIATION.

Cites

No record.

References

Cite

APA: Copy

BAHARVAND, H., & MATTHAEI, K.. (2003). IN VITRO DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS INTO FUNCTIONAL NEURONS. CELL JOURNAL (YAKHTEH), 5(19), 155-163. SID. https://sid.ir/paper/16344/en

Vancouver: Copy

BAHARVAND H., MATTHAEI K.. IN VITRO DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS INTO FUNCTIONAL NEURONS. CELL JOURNAL (YAKHTEH)[Internet]. 2003;5(19):155-163. Available from: https://sid.ir/paper/16344/en

IEEE: Copy

H. BAHARVAND, and K. MATTHAEI, “IN VITRO DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS INTO FUNCTIONAL NEURONS,” CELL JOURNAL (YAKHTEH), vol. 5, no. 19, pp. 155–163, 2003, [Online]. Available: https://sid.ir/paper/16344/en

Related Journal Papers