The Cytotoxicity of 27-Hydroxycholesterol in MCF-7 and MDA-MB-231

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Rashidi Alavijeh Mehran; Etesami Hossein; Dehghan Amin; Babajani Amirhesam; Haghjooy Javanmard Shaghayegh

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: Advanced Biomedical Research Year:2023 | Volume:12 | Issue:October Page(s): 1-6

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Title

The Cytotoxicity of 27-Hydroxycholesterol in MCF-7 and MDA-MB-231

Author(s)

Keywords

27-Hydroxycholesterol

cell viability

estrogen receptor

liver X receptor

MCF-7

MDA-MB-231

Abstract

Background: Although several roles of 27-Hydroxycholesterol (27-HC), the most abundant oxysterol in blood circulation, in cancers have been elucidated, its impact on breast cancer proliferation and its pathway remain unknown. Materials and Methods: The effect of 27-HC on breast cancer cell proliferation and its pathway was evaluated using Michigan Cancer Foundation-7 (MCF-7) and M. D. Anderson-Metastatic Breast 231 (MDA-MB-231) cell lines. The MTT assay was applied after 24-and 48-hour incubation to distinguish cell proliferation. To determine the cause of different viability results from the MTT assay, the Annexin-FITC/PI test was used at concentrations of 0. 1, 1, and 10 μM after 24-and 48-hour incubation. Results: 27-HC in concentrations of 5, 10, and 20 μM induced cell cytotoxicity compared with control. Also, the annexin V conjugated with fluorescein isothiocyanate/propidium iodide (Annexin-FITC/PI) test revealed an increase in total apoptotic cells treated with 0. 1, 1, and 10 μM of 27-HC after 48 hours (P value < 0. 05). Besides, the cytotoxic effect of 27-HC was observed at 10 μM concentration in both cell lines, MCF-7 and MDA-MB-231 (P value < 0. 05). Conclusion: The identification of 27-HC’s cytotoxic effects on both estrogen receptor (ER)-negative and ER-positive breast cancer cell lines is a novel discovery that may be linked to LXRβ.

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