EVALUATION OF ENRICHMENT, SELECTIVE AND DIFFERENTIAL MEDIA IN ISOLATION AND IDENTIFICATION OF SALMONELLA AMONG CHILDREN WITH DIARRHEA

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SOLTAN DALALL MOHAMMAD MAHDI; RAHIMI FORUSHANI ABBAS; NIKMANESH BAHRAM; TABATABAEI BAFROEI AKRAM; NUSHIN AGHILI

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Payavard Salamat Year:2011 | Volume:5 | Issue:2 Page(s): 33-41

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

5,011

Download:

Cites:

Information Journal Paper

Title

EVALUATION OF ENRICHMENT, SELECTIVE AND DIFFERENTIAL MEDIA IN ISOLATION AND IDENTIFICATION OF SALMONELLA AMONG CHILDREN WITH DIARRHEA

Author(s)

Keywords

SALMONELLAQ2

GASTROENTERITISQ3

ISOLATIONQ3

ENRICHMENTQ3

Abstract

Background and Aim: Salmonellosis is a GASTROENTERITIS caused by different serotypes of SALMONELLA and is the most common type of food poisoning in the world. The purpose of this research study is to optimize the conventional method for the ISOLATION of SALMONELLA SPP from the diarrheic specimens of children.Materials and Methods: Stool specimens were obtained from one hundred patients admitted to Children's Medical Center for diarrhea. The ENRICHMENT media were prepared by 3 methods: Rappaport Vassiliadis broth (RV), Tetrathionate broth (TT), and Selenite Cystine broth (SC). Then, for the ISOLATION of SALMONELLA SPP, the ENRICHMENT methods RV and TT were used and incubated at 42oC, and SC at 37oC. After 24 hours of incubation, the ENRICHMENT samples were inoculated into the following 6 different media: Hektone Enteric agar (HE), Rambach agar (RA), CHRO Magar Salmnella (CHROM agar SALMONELLA), Brilliant Green agar (BG), SALMONELLA-Shigella agar (SS), and Xylose-Lysine-Deoxycholate agar (XLD).Results: In total, 13 out of one hundred samples were identified as SALMONELLA SPP. All of these 13 SALMONELLA SPP samples (i. e., 100%) were positive on RV broth; the figures were 8 (61.5%) and 3 (23%) on SC and TT broths, respectively. The highest amount of ISOLATION was found by the combination of RV broth and RA agar (100%). The lowest rate, however, was obtained by the combination of TT agar and BG broth (15.4%).Conclusion: The comparison results of 3 ENRICHMENT media and 6 selective media showed that the mixture of RV broth and RA agar would be very fine for the ISOLATION of SALMONELLA SPP.

Cites

THE EFFECT OF OZONE ON BACTERIAL CONTAMINATION OF POULTRY CARCASSES AT AN INDUSTRIAL SLAUGHTERHOUSE IN TEHRAN

References

No record.

Cite

APA: Copy

SOLTAN DALALL, MOHAMMAD MAHDI, RAHIMI FORUSHANI, ABBAS, NIKMANESH, BAHRAM, TABATABAEI BAFROEI, AKRAM, & NUSHIN, AGHILI. (2011). EVALUATION OF ENRICHMENT, SELECTIVE AND DIFFERENTIAL MEDIA IN ISOLATION AND IDENTIFICATION OF SALMONELLA AMONG CHILDREN WITH DIARRHEA. PAYAVARD-SALAMAT, 5(2), 33-41. SID. https://sid.ir/paper/149868/en

Vancouver: Copy

SOLTAN DALALL MOHAMMAD MAHDI, RAHIMI FORUSHANI ABBAS, NIKMANESH BAHRAM, TABATABAEI BAFROEI AKRAM, NUSHIN AGHILI. EVALUATION OF ENRICHMENT, SELECTIVE AND DIFFERENTIAL MEDIA IN ISOLATION AND IDENTIFICATION OF SALMONELLA AMONG CHILDREN WITH DIARRHEA. PAYAVARD-SALAMAT[Internet]. 2011;5(2):33-41. Available from: https://sid.ir/paper/149868/en

IEEE: Copy

MOHAMMAD MAHDI SOLTAN DALALL, ABBAS RAHIMI FORUSHANI, BAHRAM NIKMANESH, AKRAM TABATABAEI BAFROEI, and AGHILI NUSHIN, “EVALUATION OF ENRICHMENT, SELECTIVE AND DIFFERENTIAL MEDIA IN ISOLATION AND IDENTIFICATION OF SALMONELLA AMONG CHILDREN WITH DIARRHEA,” PAYAVARD-SALAMAT, vol. 5, no. 2, pp. 33–41, 2011, [Online]. Available: https://sid.ir/paper/149868/en

Related Journal Papers