IN VITRO EVALUATION OF METHANOL EXTRACT OF AGRIMONIA EUPATORIA ON PBMC AND 12 PATHOGENIC BACTERIA

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MASJEDI M.S.; KEYHANFAR M.; BEHBAHANI M.

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Paper Information

Journal: Journal of Medicinal Plants Year:2017 | Volume:16 | Issue:SUPPLEMENT 10 Page(s): 167-173

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Title

IN VITRO EVALUATION OF METHANOL EXTRACT OF AGRIMONIA EUPATORIA ON PBMC AND 12 PATHOGENIC BACTERIA

Author(s)

MASJEDI M.S. | KEYHANFAR M. | BEHBAHANI M. | Issue Writer Certificate

Keywords

AGRIMONIA EUPATORIAQ2

ANTIBACTERIALQ2

IMMUNOSTIMULATORQ2

PBMCQ2

Abstract

Background: Today the use of medicinal plants to improve the immune system function and against pathogenic bacteria has been considered.Objective: In this study, the effect of methanol extract of the aerial parts of AGRIMONIA EUPATORIA on peripheral blood mononuclear cells (PBMC) and 12 pathogenic bacteria were investigated.Methods: The methanol extract of branches, stems, seeds and leaves of AGRIMONIA EUPATORIA were prepared and the effect of different concentrations of 50, 100, 500, 1000, 1500, 2000 and 2500 mg/ml of the extract on proliferation of PBMC was evaluated by MTT assay. Also the effect of concentrations of 1, 2, 4, 5, 7 and 10 mg/ml of the extract was tested on 12 pathogenic bacteria by disc method and on nutrient agar media.Results: The methanol extract of the branch, stem and seed of AGRIMONIA EUPATORIA showed the most stimulatory effects on immune system and induced the proliferation of PBMCs up to 8 times. Methanol extract of AGRIMONIA EUPATORIA showed ANTIBACTERIAL effects against gram-positive bacteria and the most ANTIBACTERIAL effect was on Bacillus subtilis at a concentration of 4 mg/ml and Staphylococcus aureus at concentration of 7 mg/ml.Conclusion: The AGRIMONIA EUPATORIA methanol extract showed stimulatory effects on the immune system and also ANTIBACTERIAL properties against certain gram-positive pathogenic bacteria. These finding indicate that AGRIMONIA EUPATORIA can be considered to use for immunodeficiency patients and moreover to control some bacterial infections.

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References

IMMUNOMODULATORY MEDICINAL PLANTS OF INDIA: A REVIEW

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APA: Copy

MASJEDI, M.S., KEYHANFAR, M., & BEHBAHANI, M.. (2017). IN VITRO EVALUATION OF METHANOL EXTRACT OF AGRIMONIA EUPATORIA ON PBMC AND 12 PATHOGENIC BACTERIA. JOURNAL OF MEDICINAL PLANTS, 16(SUPPLEMENT 10), 167-173. SID. https://sid.ir/paper/15390/en

Vancouver: Copy

MASJEDI M.S., KEYHANFAR M., BEHBAHANI M.. IN VITRO EVALUATION OF METHANOL EXTRACT OF AGRIMONIA EUPATORIA ON PBMC AND 12 PATHOGENIC BACTERIA. JOURNAL OF MEDICINAL PLANTS[Internet]. 2017;16(SUPPLEMENT 10):167-173. Available from: https://sid.ir/paper/15390/en

IEEE: Copy

M.S. MASJEDI, M. KEYHANFAR, and M. BEHBAHANI, “IN VITRO EVALUATION OF METHANOL EXTRACT OF AGRIMONIA EUPATORIA ON PBMC AND 12 PATHOGENIC BACTERIA,” JOURNAL OF MEDICINAL PLANTS, vol. 16, no. SUPPLEMENT 10, pp. 167–173, 2017, [Online]. Available: https://sid.ir/paper/15390/en

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