PHARMACOGNOSICAL & PLANT TISSUE CULTURE STUDIES OF ACHILLEA MILLEFOLIUM L.

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SHAMS ARDAKANI M.R.; HAJI AKHOUNDI A.; JAMSHIDI A.H.; MOHAMMAD RAFIEE P.

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Journal: Journal of Medicinal Plants Year:2006 | Volume:5 | Issue:17 Page(s): 21-26

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Information Journal Paper

Title

PHARMACOGNOSICAL & PLANT TISSUE CULTURE STUDIES OF ACHILLEA MILLEFOLIUM L.

Author(s)

SHAMS ARDAKANI M.R. | HAJI AKHOUNDI A. | JAMSHIDI A.H. | MOHAMMAD RAFIEE P. | Issue Writer Certificate

Keywords

ACHILLEA MILLEFOLIUMQ3

TISSUE CULTUREQ3

ESSENTIAL OILQ3

Abstract

Background: ACHILLEA MILLEFOLIUM L. is a traditional herbal medicine used widely as a spasmolytic, anti- cancer, anti – inflammatory and carminative. This paper focuses on the analysis of the chemical composition of ESSENTIAL OIL from the upper parts of plant and calli of ACHILLEA MILLEFOLIUM L. Method: Growing the calli of ACHILLEA MILLEFOLIUM L. and production its secondary metabolites studied and compared with those in the whole plant. Achillea seeds were first surface sterilized by shaking in 3% (W/V) aqueous hydrogen peroxide, then by shaking in 5% Na hypochlorite and 80% aqueous - ethanol solution, then under aseptic condition, strile petrydishes containing autoclaved agar (0.8%) and kept in the dark at a temprature of 25-27 oC. Seedlings were developed in two weeks. Then they were transferred to strile Murashing and Skoog (MS) culture media which were containing 2,4-D (3mg/l), IAA (5mg/l) and K (0.2mg/l) as plant grow regulators. The amorph masses (calli) were produced and subcultured every 40-45 days. Results: The result obtained from GC/MS of ESSENTIAL OIL of plant indicated that the major compounds of the ESSENTIAL OIL are alpha – Pinene, beta – Pinene and Caryophyllene oxide. Conclusion: In contrast, calli lacked ESSENTIAL OIL, this was indicated upon GC of its dichlorometanic extract. However phytochemical tests indicated that some tannins were produced by calli.

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References

ESSENTIAL OIL CONSTITUENTS OF THE IRANIAN YARROW GROWING WILD IN NORTH-EAST OF TEHRAN

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APA: Copy

SHAMS ARDAKANI, M.R., HAJI AKHOUNDI, A., JAMSHIDI, A.H., & MOHAMMAD RAFIEE, P.. (2006). PHARMACOGNOSICAL & PLANT TISSUE CULTURE STUDIES OF ACHILLEA MILLEFOLIUM L.. JOURNAL OF MEDICINAL PLANTS, 5(17), 21-26. SID. https://sid.ir/paper/15546/en

Vancouver: Copy

SHAMS ARDAKANI M.R., HAJI AKHOUNDI A., JAMSHIDI A.H., MOHAMMAD RAFIEE P.. PHARMACOGNOSICAL & PLANT TISSUE CULTURE STUDIES OF ACHILLEA MILLEFOLIUM L.. JOURNAL OF MEDICINAL PLANTS[Internet]. 2006;5(17):21-26. Available from: https://sid.ir/paper/15546/en

IEEE: Copy

M.R. SHAMS ARDAKANI, A. HAJI AKHOUNDI, A.H. JAMSHIDI, and P. MOHAMMAD RAFIEE, “PHARMACOGNOSICAL & PLANT TISSUE CULTURE STUDIES OF ACHILLEA MILLEFOLIUM L.,” JOURNAL OF MEDICINAL PLANTS, vol. 5, no. 17, pp. 21–26, 2006, [Online]. Available: https://sid.ir/paper/15546/en

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