Comparison of Vitek 2C antifungal susceptibility testing with broth microdilution testing for Candida species

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Sudhaharan Sukanya; Raju Sundarapu Naga Appala; Pamidimukkala Umabala

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Journal: Medical Laboratory Journal Year:2024 | Volume:18 | Issue:5 Page(s): 4-7

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Title

Comparison of Vitek 2C antifungal susceptibility testing with broth microdilution testing for Candida species

Author(s)

Sudhaharan Sukanya | Raju Sundarapu Naga Appala | Pamidimukkala Umabala | Issue Writer Certificate

Keywords

Antifungal susceptibility testing

Candida

Broth microdilution

Abstract

Background: The reference method for Antifungal susceptibility testing is Broth microdilution according to Clinical and Laboratory Standard Institute (CLSI) guidelines. However, the fully automated system, Vitek 2C system may reduce the workload and observer bias associated with manual Broth microdilution. This study aimed to compare the results of YS08 card with the results of the Broth microdilution (BMD) method. Methods: A total of 50 clinical Candida isolates were included in the study. The susceptibility testing was done by Vitek 2C using the YS08 card. Broth microdilution was done according to CLSI guidelines M27M44S-Ed3. Results: For C. albicans, the categorical agreement was 85.8%, 71.5%, 85.8%, and 100% for fluconazole, voriconazole, caspofungin, and micafungin, respectively. The minor errors (MiE) of 14.2% for fluconazole and caspofungin, 28.5% for voriconazole, were detected in C. albicans. In C. glabrata, the categorical agreement (CA) was 100% for micafungin, voriconazole, but 63.7% for caspofungin. An MiE of 36.3% was detected for caspofungin. C. parapsilosis showed a 100% CA for fluconazole, caspofungin, and micafungin, and 85.8% for voriconazole. There were 14.2% of MiEs for voriconazole. In C. tropicalis, 100 % CA was observed for fluconazole, micafungin, and caspofungin, and 88.9% for voriconazole. Moreover, 11.1% (1/9) of MiEs was observed for voriconazole. In C. auris, there was a 100% CA for caspofungin and micafungin, 77.8% for fluconazole, and 66.7% for amphotericin B. There was a major error of 22.2% for fluconazole and 33.3% for amphotericin B. Conclusion: The majority of Vitek 2C showed comparable results with the Broth microdilution (BMD) method. Only minor errors (MiEs) were observed in the tested Candida species.

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