EVALUATING THE EXPRESSION OF OCT-4, NANOG, SOX2 AND NUCLEOSTEMIN IN COLON CANCER CELL LINES (CACO-2 AND HT-29)

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AMINI SABRIEH; FATHI FARDIN; PARIVAR KAZEM; MOHSENI KOUCHESFAHANI HOMA; REZAEI MOHAMMAD JAFAR; NIKKHOU BAHRAM

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Journal: Cell Journal (Yakhteh) Year:2010 | Volume:12 | Issue:2 (46) Page(s): 223-230

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Title

EVALUATING THE EXPRESSION OF OCT-4, NANOG, SOX2 AND NUCLEOSTEMIN IN COLON CANCER CELL LINES (CACO-2 AND HT-29)

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CANCER STEM CELLQ1

SELF-RENEWALQ1

ADENOCARCINOMAQ1

COLORECTALQ1

Abstract

Objective: Evaluating the expression of Oct-4, NANOG, Sox2 and Nucleostemin in colon cancer cell lines (Caco-2 and HT-29).Materials and Methods: Caco-2 and HT-29 human colon cancer cell lines were cultured in Dulbecco’s modified eagles medium (DMEM) and Roswell Park Memorial Institute medium (RPMI) respectively, containing 10% fetal bovin serum (FBS) with 1% peniciline and streptomycinen in 37o, 5% CO2 incubator. Total RNA was isolated using the ISOGEN method. RNA integrity was checked with the use of agarose gel electrophoresis and spectrophotometry. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to examin the samples. The expression of Oct-4 and Nucleostemin at the protein level was further determined using immunocytochemistry.Results: RT-PCR analysis of Caco2 and HT-29 colon cancer cell lines showed expression of Oct-4, NANOG, Sox2 and Nucleostemin genes . Also immunocytochemical analysis confirmed the cytoplasmic and nuclear expression of the Oct-4 protein and Nucleostemin proteins.Conclusion: Collectively, our data confirmed the expression of Oct-4, NANOG, Sox2 and Nucleostemin in colon cancer cells and suggested that their expression can be used as potential tumor markers in diagnosis and /or prognosis of colon tumors. These results confirm the potential value of the cancer stem-cell theory in cancer therapy.

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APA: Copy

AMINI, SABRIEH, FATHI, FARDIN, PARIVAR, KAZEM, MOHSENI KOUCHESFAHANI, HOMA, REZAEI, MOHAMMAD JAFAR, & NIKKHOU, BAHRAM. (2010). EVALUATING THE EXPRESSION OF OCT-4, NANOG, SOX2 AND NUCLEOSTEMIN IN COLON CANCER CELL LINES (CACO-2 AND HT-29). CELL JOURNAL (YAKHTEH), 12(2 (46)), 223-230. SID. https://sid.ir/paper/16196/en

Vancouver: Copy

AMINI SABRIEH, FATHI FARDIN, PARIVAR KAZEM, MOHSENI KOUCHESFAHANI HOMA, REZAEI MOHAMMAD JAFAR, NIKKHOU BAHRAM. EVALUATING THE EXPRESSION OF OCT-4, NANOG, SOX2 AND NUCLEOSTEMIN IN COLON CANCER CELL LINES (CACO-2 AND HT-29). CELL JOURNAL (YAKHTEH)[Internet]. 2010;12(2 (46)):223-230. Available from: https://sid.ir/paper/16196/en

IEEE: Copy

SABRIEH AMINI, FARDIN FATHI, KAZEM PARIVAR, HOMA MOHSENI KOUCHESFAHANI, MOHAMMAD JAFAR REZAEI, and BAHRAM NIKKHOU, “EVALUATING THE EXPRESSION OF OCT-4, NANOG, SOX2 AND NUCLEOSTEMIN IN COLON CANCER CELL LINES (CACO-2 AND HT-29),” CELL JOURNAL (YAKHTEH), vol. 12, no. 2 (46), pp. 223–230, 2010, [Online]. Available: https://sid.ir/paper/16196/en

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