STUDY OF IMMUNOTHERAPY WITH ENDOGENOUS OPIOD (MET-ENKEPHALIN) ACTIVATED TUMOR INFILTRATING LYMPHOCYTES IN FIBROSARCOMA INDUCED BALB/C MICE

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AMINI ABAS ALI; HAJATI JAMSHID; VOJGANI MOHAMMAD; GHEFLATI ZAHRA; NAMDAR A.; HOLAKOUEI M.; KHANSARI NEMAT ELAH

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Journal: Cell Journal (Yakhteh) Year:2010 | Volume:11 | Issue:4 (44) Page(s): 408-417

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Title

STUDY OF IMMUNOTHERAPY WITH ENDOGENOUS OPIOD (MET-ENKEPHALIN) ACTIVATED TUMOR INFILTRATING LYMPHOCYTES IN FIBROSARCOMA INDUCED BALB/C MICE

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MET-ENK COULD BE A POTENTIAL NEW FACTOR FOR ACTIVATING LYMPHOCYTES IN VITROQ1

Abstract

Objective: In this study the effects of met-enkephalin on tumor infiltrating lymphocytes for cancer treatment in fibrosarcoma bearing mice was evaluated.Materials and Methods: Initially, to obtain the most effective dose and treating time for the induction of CD25, splenocytes were cultured with several doses of met-enkephalin. Flowcytometry was used to evaluate CD25 expression. The best dose and treating time were used to stimulate tumor infiltrating lymphocytes (TILs). To obtain pure CD4+ and CD8+ cells, TILs were taken from tumors by enzymatic tissue disaggregation and purified by magnet bead cell separation. After TILs stimulation they were re-injected into three groups of other fibrosarcoma bearing mice. The first group received only CD4+ TILs, the second group received only CD8+ TILs, and the third group received both CD4+ and CD8+ TILs. A fourth group that served as the control group received only phosphate buffered saline (PBS). The effect of this treatment on tumor volume, mice survival, effector cells, regulatory T cells and serum level Bcl-2were evaluated. To analyze data in both the experimental and control groups one way ANOVA was used followed by the Tukey test. P value<0.05 was considered significant.Results: Treatment with met-enkephalin at a dose of 10-10 M for 6 hours was most effective in CD25 induction on the splenocytes of Balb/C mice. There were a significant decrease in tumors growth in both the CD8+ and CD4+ activated TILs injected groups (p<0.044 and p<0.017, respectively). The result of the CD4+ plus CD8+ activated TILs injected group was not significantly different from control group (p<0.661). There was an improvement in survival amongst the mice in all treated groups (p<0.001 for all three groups). FoxP3 levels in all groups were significantly low (p<0.001, p<0.002 and p<0.001 for the CD4+, CD8+ and CD4+ plus CD8+ activated TILs injected groups, respectively). CD25 and Bcl-2 expressions were higher in the treated groups, but only the CD4+ activated TILs injected group was significant (p<0.002 for CD25, p<0.001 for Bcl-2).

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APA: Copy

AMINI, ABAS ALI, HAJATI, JAMSHID, VOJGANI, MOHAMMAD, GHEFLATI, ZAHRA, NAMDAR, A., HOLAKOUEI, M., & KHANSARI, NEMAT ELAH. (2010). STUDY OF IMMUNOTHERAPY WITH ENDOGENOUS OPIOD (MET-ENKEPHALIN) ACTIVATED TUMOR INFILTRATING LYMPHOCYTES IN FIBROSARCOMA INDUCED BALB/C MICE. CELL JOURNAL (YAKHTEH), 11(4 (44)), 408-417. SID. https://sid.ir/paper/16219/en

Vancouver: Copy

AMINI ABAS ALI, HAJATI JAMSHID, VOJGANI MOHAMMAD, GHEFLATI ZAHRA, NAMDAR A., HOLAKOUEI M., KHANSARI NEMAT ELAH. STUDY OF IMMUNOTHERAPY WITH ENDOGENOUS OPIOD (MET-ENKEPHALIN) ACTIVATED TUMOR INFILTRATING LYMPHOCYTES IN FIBROSARCOMA INDUCED BALB/C MICE. CELL JOURNAL (YAKHTEH)[Internet]. 2010;11(4 (44)):408-417. Available from: https://sid.ir/paper/16219/en

IEEE: Copy

ABAS ALI AMINI, JAMSHID HAJATI, MOHAMMAD VOJGANI, ZAHRA GHEFLATI, A. NAMDAR, M. HOLAKOUEI, and NEMAT ELAH KHANSARI, “STUDY OF IMMUNOTHERAPY WITH ENDOGENOUS OPIOD (MET-ENKEPHALIN) ACTIVATED TUMOR INFILTRATING LYMPHOCYTES IN FIBROSARCOMA INDUCED BALB/C MICE,” CELL JOURNAL (YAKHTEH), vol. 11, no. 4 (44), pp. 408–417, 2010, [Online]. Available: https://sid.ir/paper/16219/en

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