THE EFFECTS OF T-CELL CONDITIONED MEDIA ON THE INDUCTION OF DENDRITIC CELL (DC1) MATURATION FOR EFFECTIVE TUMOR IMMUNOTHERAPY

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ASADI MASOUMEH; FAROKHI FARAH; GANJI BAKHSH MEYSAM; DELIREZH NOWRUZ; NEJATI VAHID; GHOLAMI KEYKAVOS

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Journal: TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ) Year:2011 | Volume:69 | Issue:1 Page(s): 1-8

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Title

THE EFFECTS OF T-CELL CONDITIONED MEDIA ON THE INDUCTION OF DENDRITIC CELL (DC1) MATURATION FOR EFFECTIVE TUMOR IMMUNOTHERAPY

Author(s)

Keywords

CONDITIONED MEDIAQ2

DENDRITIC CELLSQ2

T-LYMPHOCYTEQ2

TUMOR IMMUNOTHERAPYQ2

Abstract

Background: Nowadays, DENDRITIC CELLS (DC) are used for TUMOR IMMUNOTHERAPY as they can induce immune responses against tumor cells. In this research, we comprehensively studied the maturation stimulus addition, PHA-activated T-cell (PHATCM) conditioned medium, autologous monocyte-conditioned medium (MCM) and TNF-á for their ability to promote uniformly mature DENDRITIC CELLS that elicit T-cell responses.Methods: Plastic adherent monocytes were cultured with granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4) for five days and two days with monocyte-conditioned medium (MCM), tumor necrotizing factor-á (TNF-á) without TCM (PHA-activated T-cell conditioned medium). Phenotypic and functional analyses were carried out using anti-CD14, anti-CD80, anti-CD86, anti-CD83 monoclonal antibodies. Phagocytic activity, mixed lymphocyte reaction (MLR) and cytokine production were also evaluated.Results: The generated DENDRITIC CELLS had high expression of surface molecules i.e. CD80, CD83, CD86 and HLA-DR. Moreover, the cells had low phagocytic and high Tlymphocyte stimulating activities. Measurement of the produced cytokines showed the generation of type-1 DENDRITIC CELLS (DC1) in the study.Conclusion: The findings indicated that more efficient maturation of DENDRITIC CELLS could be achieved by the use of PHA-activated T-LYMPHOCYTE conditioned medium in the culture medium. The aforesaid supernatant can be used as a maturation factor for the production of efficient DENDRITIC CELLS with the ability to be used for TUMOR IMMUNOTHERAPY. This conditioned medium can provide new strategies and evolve into more advance tools for the generation of DENDRITIC CELLS in vitro for TUMOR IMMUNOTHERAPY.

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APA: Copy

ASADI, MASOUMEH, FAROKHI, FARAH, GANJI BAKHSH, MEYSAM, DELIREZH, NOWRUZ, NEJATI, VAHID, & GHOLAMI, KEYKAVOS. (2011). THE EFFECTS OF T-CELL CONDITIONED MEDIA ON THE INDUCTION OF DENDRITIC CELL (DC1) MATURATION FOR EFFECTIVE TUMOR IMMUNOTHERAPY. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 69(1), 1-8. SID. https://sid.ir/paper/38666/en

Vancouver: Copy

ASADI MASOUMEH, FAROKHI FARAH, GANJI BAKHSH MEYSAM, DELIREZH NOWRUZ, NEJATI VAHID, GHOLAMI KEYKAVOS. THE EFFECTS OF T-CELL CONDITIONED MEDIA ON THE INDUCTION OF DENDRITIC CELL (DC1) MATURATION FOR EFFECTIVE TUMOR IMMUNOTHERAPY. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2011;69(1):1-8. Available from: https://sid.ir/paper/38666/en

IEEE: Copy

MASOUMEH ASADI, FARAH FAROKHI, MEYSAM GANJI BAKHSH, NOWRUZ DELIREZH, VAHID NEJATI, and KEYKAVOS GHOLAMI, “THE EFFECTS OF T-CELL CONDITIONED MEDIA ON THE INDUCTION OF DENDRITIC CELL (DC1) MATURATION FOR EFFECTIVE TUMOR IMMUNOTHERAPY,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 69, no. 1, pp. 1–8, 2011, [Online]. Available: https://sid.ir/paper/38666/en

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