DETERMINATION OF DISTRIBUTION OF ICSA GENE AND ICSA PROTEIN BANDS BETWEEN SHIGELLA FLEXNERI ISOLATED FROM 3 HOSPITALS IN TEHRAN

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HAKEMI VALA M.; NOUROUZI JAMILEH; KAZEMI B.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2006 | Volume:7 | Issue:4 (28) Page(s): 250-253

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

827

Download:

257

Cites:

Information Journal Paper

Title

DETERMINATION OF DISTRIBUTION OF ICSA GENE AND ICSA PROTEIN BANDS BETWEEN SHIGELLA FLEXNERI ISOLATED FROM 3 HOSPITALS IN TEHRAN

Author(s)

HAKEMI VALA M. | NOUROUZI JAMILEH | KAZEMI B. | Issue Writer Certificate

Keywords

SHIGELLA FLEXNERIQ2

ICSA GENEQ2

ICSA PROTEINQ3

PCRQ2

SDS-PAGEQ2

Abstract

Introduction: Shigella is a facultative intracellular pathogen that uses the host actin cytoskeleton protein for intra- and intercellular spread. The aim of this study was to determine the distribution of ICSA GENE and IcsA expressed protein bands among SHIGELLA FLEXNERI strains isolated from 3 clinical centers in Tehran. Material and Methods: Two hundred and seventy five isolated SHIGELLA FLEXNERI strains were identified by standard microbiological and biochemical methods. DNA isolation was performed using sodium perchlorate method. Hot start-PCR was done with 2 pairs of primers and the products were separated through agarose gel (0.8%) in TAE buffer. DNA fragments were visualized by ethidium bromide staining under UV illumination. Whole membrane preparation was used to examine the protein profiles and identification of probable IcsA (120-kda) protein band by SDS-PAGE. Results: From 100 isolated SHIGELLA FLEXNERI strains, both bands of 1600 bp and 1709 bp were detected in 46 isolates (46%). A 120 kDa band which seems to be related to ICSA PROTEIN was detected in 46 isolates (46%). The protein bands varied between 30 and 150 kDa. Discussion: IcsA is both necessary and sufficient for actin assembly in SHIGELLA FLEXNERI. Since ICSA GENE and ICSA PROTEIN band were not found in all Shigella strains, it seems that not all strains have the same pathogenesis. On the other hand, since the demonstration of ICSA GENE by PCR in all Shigella strains (46%) corresponded to the presence of a 120 kDa protein band by SDS-PAGE (46%), it seems that both tests may confirm each other. However, the PCR may be more accurate than SDS-PAGE.

Cites

No record.

References

Cite

APA: Copy

HAKEMI VALA, M., NOUROUZI, JAMILEH, & KAZEMI, B.. (2006). DETERMINATION OF DISTRIBUTION OF ICSA GENE AND ICSA PROTEIN BANDS BETWEEN SHIGELLA FLEXNERI ISOLATED FROM 3 HOSPITALS IN TEHRAN. CELL JOURNAL (YAKHTEH), 7(4 (28)), 250-253. SID. https://sid.ir/paper/16373/en

Vancouver: Copy

HAKEMI VALA M., NOUROUZI JAMILEH, KAZEMI B.. DETERMINATION OF DISTRIBUTION OF ICSA GENE AND ICSA PROTEIN BANDS BETWEEN SHIGELLA FLEXNERI ISOLATED FROM 3 HOSPITALS IN TEHRAN. CELL JOURNAL (YAKHTEH)[Internet]. 2006;7(4 (28)):250-253. Available from: https://sid.ir/paper/16373/en

IEEE: Copy

M. HAKEMI VALA, JAMILEH NOUROUZI, and B. KAZEMI, “DETERMINATION OF DISTRIBUTION OF ICSA GENE AND ICSA PROTEIN BANDS BETWEEN SHIGELLA FLEXNERI ISOLATED FROM 3 HOSPITALS IN TEHRAN,” CELL JOURNAL (YAKHTEH), vol. 7, no. 4 (28), pp. 250–253, 2006, [Online]. Available: https://sid.ir/paper/16373/en

Related Journal Papers