CONSTRUCTION OF PDS132:: ΔICSA FOR TARGETED DELETION OF A REGION OF ICSA GENE IN ORDER TO GENERATE A LIVE ATTENUATED SHIGELLA VACCINE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HOSSEINI S.M.; SAADATI M.; YAKHCHALY M.B.; NAYERI FASAEI B.; AHMADIDANESH H.; MIRZAEI M.; BAGHERY K.; ZARE M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Arak University Medical Sciences Year:2012 | Volume:15 | Issue:2 (61) Page(s): 35-47

Download Full-Text

Persian Verion

View:

829

Download:

Cites:

Information Journal Paper

Title

CONSTRUCTION OF PDS132:: ΔICSA FOR TARGETED DELETION OF A REGION OF ICSA GENE IN ORDER TO GENERATE A LIVE ATTENUATED SHIGELLA VACCINE

Author(s)

Keywords

ALLELIC EXCHANGEQ2

ICSAQ2

SHIGELLA DYSENTERIAE TYPE 1Q2

SHIGELLOSISQ2

SUICIDE VECTORQ2

Abstract

Background: Live attenuated Shigella vaccines have shown promise in inducing protective immune responses in human clinical trials. The aim of this study was to design and construct pDS132::ΔicsA as a suicide plasmid for targeted deletion of a region of ICSA gene in Shigella.Materials and Methods: In this experimental study, species and serotypes of Shigella isolated from diarrhea samples of children at Firozabadi and Milad Hospitals of Tehran were confirmed by using serological and PCR tests. Identification primers of ICSA gene were designed and then cloned to the pGEM-5zf vector and sequenced. According to ICSA restriction enzyme map, 1751 bp of ICSA gene was deleted by HincII restriction enzyme and the DicsA was constructed successfully. The pGEMDicsA vector was digested by use of SphI and SalI enzymes and was then cloned to a SUICIDE VECTOR (pSD132). Precision of the process was confirmed by phenotype and genotype tests.Results: The SHIGELLA DYSENTERIAE TYPE 1 strain was verified by serological tests and PCR. Sequence of the ICSA gene in the native strain was identical to the strains submitted in the gene-bank database. Since the pDS132::DicsA contains 1484 bp derived from ICSA gene, it can be used to disrupt ICSA gene as a specific SUICIDE VECTOR.Conclusion: Application of suicide systems facilitated mutant construction in more specific and effective methods in comparison with the other primary techniques such as serial passage.

Cites

No record.

References

No record.

Cite

APA: Copy

HOSSEINI, S.M., SAADATI, M., YAKHCHALY, M.B., NAYERI FASAEI, B., AHMADIDANESH, H., MIRZAEI, M., BAGHERY, K., & ZARE, M.. (2012). CONSTRUCTION OF PDS132:: ΔICSA FOR TARGETED DELETION OF A REGION OF ICSA GENE IN ORDER TO GENERATE A LIVE ATTENUATED SHIGELLA VACCINE. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 15(2 (61)), 35-47. SID. https://sid.ir/paper/384026/en

Vancouver: Copy

HOSSEINI S.M., SAADATI M., YAKHCHALY M.B., NAYERI FASAEI B., AHMADIDANESH H., MIRZAEI M., BAGHERY K., ZARE M.. CONSTRUCTION OF PDS132:: ΔICSA FOR TARGETED DELETION OF A REGION OF ICSA GENE IN ORDER TO GENERATE A LIVE ATTENUATED SHIGELLA VACCINE. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2012;15(2 (61)):35-47. Available from: https://sid.ir/paper/384026/en

IEEE: Copy

S.M. HOSSEINI, M. SAADATI, M.B. YAKHCHALY, B. NAYERI FASAEI, H. AHMADIDANESH, M. MIRZAEI, K. BAGHERY, and M. ZARE, “CONSTRUCTION OF PDS132:: ΔICSA FOR TARGETED DELETION OF A REGION OF ICSA GENE IN ORDER TO GENERATE A LIVE ATTENUATED SHIGELLA VACCINE,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 15, no. 2 (61), pp. 35–47, 2012, [Online]. Available: https://sid.ir/paper/384026/en

Related Journal Papers