STUDYING THE ACTIVITY OF FIBROBLAST GROWTH FACTOR 18 AND UROKINASE PLASMINOGEN ACTIVATOR RECEPTOR PROMOTERS IN TWO COLON CANCER CELL LINES

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TEYMOURI TOULABI L.; AZADMANESH K.; JAMALI SOMAYEH; AMANZADEH A.; KARIMIPOUR MORTEZA; ZEYNALI SIROUS

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Journal: Cell Journal (Yakhteh) Year:2009 | Volume:11 | Issue:2 (42) Page(s): 142-153

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Title

STUDYING THE ACTIVITY OF FIBROBLAST GROWTH FACTOR 18 AND UROKINASE PLASMINOGEN ACTIVATOR RECEPTOR PROMOTERS IN TWO COLON CANCER CELL LINES

Author(s)

Keywords

COLONQ1

NEOPLASMQ1

UROKINASE PLASMINOGEN ACTIVATOR RECEPTORQ1

FIBROBLAST GROWTH FACTOR 18Q1

PROMOTER REGIONSQ1

Abstract

Introduction: Wnt and k-ras are two main signaling pathways activated in COLON cancer. Many genes are upregulated downstream of these signaling pathways. The aim of this study was to assess the activity of Wnt and k-ras in HCT116 and SW480 cell lines by making two reporters constructs using promoters downstream of these pathways (fibroblast growth factor18 [FGF18] and UROKINASE PLASMINOGEN ACTIVATOR RECEPTOR [UPAR]).Materials and Methods: UPARLacZ, FGF18LacZ, negative (pUCLacZ) and positive (CMVLacZ) control plasmids and pRc/CMV2CAT were constructed. Expressions of LacZ in both cell lines were studied by bgal staining and ELISA after normalization with CAT expression.Results: In both cell lines, FGF18LacZ transfected cells stained more than UPARLacZ transfected ones. This difference was more prominent in SW480. Both constructs have the ability of expression in both cell lines. It was also proven that FGF18LacZ was significantly more active than UPARLacZ in both cell lines. Expression of FGF18LacZ in HCT116 and SW480 cell lines was respectively 1.34 and 4.4 times more than UPARLacZ.Conclusion: Despite the fact that in HCT116 the Ras pathway is activated, FGF18LacZ is more active than UPARLacZ although the UPAR promoter is more active in HCT116 cell line than SW480 cell line. These findings are in accordance with previous studies that in all COLON cancer cell lines Wnt signaling pathway is active even though there is no mutation in any part of it. Wnt is the main signaling pathway responsible for carcinogenesis in COLON epithelial cells. These constructs can be used as reporters for studying the above mentioned signaling pathways in other cell lines.

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APA: Copy

TEYMOURI TOULABI, L., AZADMANESH, K., JAMALI, SOMAYEH, AMANZADEH, A., KARIMIPOUR, MORTEZA, & ZEYNALI, SIROUS. (2009). STUDYING THE ACTIVITY OF FIBROBLAST GROWTH FACTOR 18 AND UROKINASE PLASMINOGEN ACTIVATOR RECEPTOR PROMOTERS IN TWO COLON CANCER CELL LINES. CELL JOURNAL (YAKHTEH), 11(2 (42)), 142-153. SID. https://sid.ir/paper/16406/en

Vancouver: Copy

TEYMOURI TOULABI L., AZADMANESH K., JAMALI SOMAYEH, AMANZADEH A., KARIMIPOUR MORTEZA, ZEYNALI SIROUS. STUDYING THE ACTIVITY OF FIBROBLAST GROWTH FACTOR 18 AND UROKINASE PLASMINOGEN ACTIVATOR RECEPTOR PROMOTERS IN TWO COLON CANCER CELL LINES. CELL JOURNAL (YAKHTEH)[Internet]. 2009;11(2 (42)):142-153. Available from: https://sid.ir/paper/16406/en

IEEE: Copy

L. TEYMOURI TOULABI, K. AZADMANESH, SOMAYEH JAMALI, A. AMANZADEH, MORTEZA KARIMIPOUR, and SIROUS ZEYNALI, “STUDYING THE ACTIVITY OF FIBROBLAST GROWTH FACTOR 18 AND UROKINASE PLASMINOGEN ACTIVATOR RECEPTOR PROMOTERS IN TWO COLON CANCER CELL LINES,” CELL JOURNAL (YAKHTEH), vol. 11, no. 2 (42), pp. 142–153, 2009, [Online]. Available: https://sid.ir/paper/16406/en

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