IMMUNOLOCALIZATION OF NA+, K+- ATPASE AND IONOCYTES IN 
THE GILLS OF CATFISH, SILURUS GLANIS

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KHODABANDEH S.; TAGHIZADEH ZAHRA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: Cell Journal (Yakhteh) Year:2006 | Volume:8 | Issue:1 (29) Page(s): 45-52

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Title

IMMUNOLOCALIZATION OF NA+, K+- ATPASE AND IONOCYTES IN THE GILLS OF CATFISH, SILURUS GLANIS

Author(s)

KHODABANDEH S. | TAGHIZADEH ZAHRA | Issue Writer Certificate

Keywords

NA+Q2

K+-ATPASEQ2

IONOCYTEQ2

SILURUS GLANISQ2

OSMOREGULATIONQ1

IMMUNOHISTOCHEMISTRYQ2

Abstract

Introduction: The regulation of the body fluid content (osmoregulation) of an aquatic animal, is performed by several organs. In fish, osmoregulatory mechanisms are based on the function of specialized cells (ionocytes) located in various tissues and organs including gills. Na+, K+-ATPase is one of the main osmoregulatory enzymes enabling the use of ATP as a source of energy for ion transport through a Na+, K+ exchange pump cross epithelial membranes of IONOCYTEs. The aim of this study was localization of this enzyme and IONOCYTEs in the gills of S. glanis.Material and Methods: For light microscopic observation, samples were fixed in Buin for 24 h dehydrated with ethanol, and embedded in paraffin. Serial sections, 5 µm thick, were stained by the Haematoxylin, Eosin and Methyl green. Immunolocalization of the Na+, K+-ATPase was performed by immunofluorescence light microscopy with a Mouse Monoclonal Antibody IgGα5 raised against the α-subunit of he Chicken Na+, K+-ATPase (Developmental Studies Hybridoma Bank, University of Iowa , USA) and a Mouse Anti-fluorescein Antibody FITC (Jackson Immuno Research , USA).Results: In longitudinal sections of the gill, two series of lamellae were observed on both sides of the filaments. Gill filaments and lamellae were lined by special cells, contained pavement cells, mucous cells and IONOCYTEs. The IONOCYTEs were located in the basal parts of lamellae, inter-lamellar regions and in the apical parts of gill filaments.The IONOCYTEs showed a ovoid-spheroid shape with a strong immunofluorescence of Na+, K+-ATPase in the baso-lateral regions. Conclusion: In catfish, S. glanis, the IONOCYTEs was found to be distributed on filaments and mainly in inter-lamellar regions. Na+, K+-ATPase enzyme with a highly consistent immunoreactivity was observed in the baso-lateral parts of the IONOCYTEs. These findings show that, in S. glanis, the filaments appear as the main site of osmoregulation and the gill lamellae are mainly devoted to respiration.

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APA: Copy

KHODABANDEH, S., & TAGHIZADEH, ZAHRA. (2006). IMMUNOLOCALIZATION OF NA⁺, K⁺- ATPASE AND IONOCYTES IN THE GILLS OF CATFISH, SILURUS GLANIS. CELL JOURNAL (YAKHTEH), 8(1 (29)), 45-52. SID. https://sid.ir/paper/16465/en

Vancouver: Copy

KHODABANDEH S., TAGHIZADEH ZAHRA. IMMUNOLOCALIZATION OF NA⁺, K⁺- ATPASE AND IONOCYTES IN THE GILLS OF CATFISH, SILURUS GLANIS. CELL JOURNAL (YAKHTEH)[Internet]. 2006;8(1 (29)):45-52. Available from: https://sid.ir/paper/16465/en

IEEE: Copy

S. KHODABANDEH, and ZAHRA TAGHIZADEH, “IMMUNOLOCALIZATION OF NA⁺, K⁺- ATPASE AND IONOCYTES IN THE GILLS OF CATFISH, SILURUS GLANIS,” CELL JOURNAL (YAKHTEH), vol. 8, no. 1 (29), pp. 45–52, 2006, [Online]. Available: https://sid.ir/paper/16465/en

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