GROWTH ENHACEMENT OF L. PNEUMOPHILA BY CO-CULTIVATION WITH AMOEBAE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HOSSEINIDOUST S.R.; MOBAREZ A.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2000 | Volume:2 | Issue:5 Page(s): 13-17

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

971

Download:

Cites:

Information Journal Paper

Title

GROWTH ENHACEMENT OF L. PNEUMOPHILA BY CO-CULTIVATION WITH AMOEBAE

Author(s)

HOSSEINIDOUST S.R. | MOBAREZ A. | Issue Writer Certificate

Keywords

L. PNEUMOPHILAQ4

ENRICHEMENTQ4

A. CASTELLANIIQ4

RFLPQ4

Abstract

Introduction: The suspected water samples contaminated with Legionella were enriched with Acanthamoebae. Small numbers of bacteria (not detectable by culture) can be multiplied in Amoebae up to culturable level in co-culture condition. The conventional culture method for legionnaires' disease bacterium always suffers from low sensitivity. This co-culture method can significantly improve culture sensitivity. Materials and Methods: Legionella spp. generally benefits from interacting with free living amoebae in an aquatic environment. Acanthamoebae (the natural host of the legionella) was used for enrichment of preliminary culture-negative samples. The enriched samples were then cultured again on a supplemented selective media, then were confirmed with the agglutination test, direct immunofluorescence and restriction fragment length polymorphism. Results: L. PNEUMOPHILA were isolated from 15% of the samples and confirmed by biochemical tests as well as legionella latex agglutination test and then grouped by direct fluorescent antibody and restriction fragment length ploymorphism. Conclusion: In fact, this method has enhanced the bacterial multiplication (in original sample prior to culturing). We assume that all negative samples encountered in legionella laboratories should be submitted for additional culture after preliminary enrichment with Acanthamoebae castellanii.

Cites

No record.

References

Cite

APA: Copy

HOSSEINIDOUST, S.R., & MOBAREZ, A.. (2000). GROWTH ENHACEMENT OF L. PNEUMOPHILA BY CO-CULTIVATION WITH AMOEBAE. CELL JOURNAL (YAKHTEH), 2(5), 13-17. SID. https://sid.ir/paper/16514/en

Vancouver: Copy

HOSSEINIDOUST S.R., MOBAREZ A.. GROWTH ENHACEMENT OF L. PNEUMOPHILA BY CO-CULTIVATION WITH AMOEBAE. CELL JOURNAL (YAKHTEH)[Internet]. 2000;2(5):13-17. Available from: https://sid.ir/paper/16514/en

IEEE: Copy

S.R. HOSSEINIDOUST, and A. MOBAREZ, “GROWTH ENHACEMENT OF L. PNEUMOPHILA BY CO-CULTIVATION WITH AMOEBAE,” CELL JOURNAL (YAKHTEH), vol. 2, no. 5, pp. 13–17, 2000, [Online]. Available: https://sid.ir/paper/16514/en

Related Journal Papers